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(Plasmid #190243)


Item Catalog # Description Quantity Price (USD)
Plasmid 190243 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Total vector size (bp) 5783
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
  • gRNA/shRNA sequence
  • Species
    E. coli

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    gRNA obtained from pgRNA (Addgene #44251), LacI from pMJ842 (Addgene #39318), and backbone derived from pSKunk-BLA (Addgene #61531).

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSelect-6 was a gift from Marc Ostermeier (Addgene plasmid # 190243 ; ; RRID:Addgene_190243)
  • For your References section:

    A bacterial dual positive and negative selection system for dCas9 activity. Spisak S, O'Brien B, Ostermeier M. PLoS One. 2022 Jun 3;17(6):e0269270. doi: 10.1371/journal.pone.0269270. eCollection 2022. 10.1371/journal.pone.0269270 PubMed 35657952