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Zinc cluster transcription factors frequently activate target genes using a non-canonical half-site binding mode.

Recio PS, Mitra NJ, Shively CA, Song D, Jaramillo G, Lewis KS, Chen X, Mitra RD
Nucleic Acids Res. 2023 Jun 9;51(10):5006-5021. doi: 10.1093/nar/gkad320. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
190582ADH1pro_LEU3_SIR4Transcription factor tagged with Sir4 used for Calling Cards experiments for binding measurement
190583ADH1pro_LYS14_SIR4Transcription factor tagged with Sir4 used for Calling Cards experiments for binding measurement
190585ADH1pro_YRR1_SIR4Transcription factor tagged with Sir4 used for Calling Cards experiments for binding measurement
190586ADH1pro_TEA1_SIR4Transcription factor tagged with Sir4 used for Calling Cards experiments for binding measurement
190588TEF2pro_mCherry_YFPDual reporter backbone plasmid for CCRA experiment- LEU2 Marker

Antibodies from Article