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Dual-specificity RNA aptamers enable manipulation of target-specific O-GlcNAcylation and unveil functions of O-GlcNAc on beta-catenin.
Zhu Y, Hart GW
Cell. 2022 Dec 30:S0092-8674(22)01529-X. doi: 10.1016/j.cell.2022.12.016. PubMed Article

Plasmids from Article

ID Plasmid Purpose
190821pET24a-ncOGT-FLFor expression of full-length human ncOGT in E.coli. The ncOGT is 6x His-tagged on its N-terminus and codon-optimized for E.coli.
190822pET24a-ncOGT-TPRFor expression of the TPR domain of human ncOGT (C467X mutation) in E.coli. The ncOGT is 6x His-tagged on its N-terminus and codon-optimized for E.coli.
190824pEGFP-β-catenin_S23A,T40A,T41A,T112AFor mammalian expression of β-catenin with 4 O-GlcNAc sites mutated (S23A, T40A, T41A and T112A) and GFP-tag on its N-terminus.
190825pSilencer2.1-U6-T1For mammalian expression of T1, the RNA aptamer of ncOGT. Expression is driven by a U6 promoter.
190826pSilencer2.1-U6-AP3For mammalian expression of AP3, the RNA aptamer of GFP. Expression is driven by a U6 promoter.
190827pSilencer2.1-U6-17-6For mammalian expression of 17-6, a non-aptamer RNA. Expression is driven by a U6 promoter.
190828pSilencer2.1-U6-T1-U6-AP3For mammalian expression of T1 and AP3, the RNA aptamers of ncOGT and GFP, respectively. Expression of each aptamer is driven by a U6 promoter.
190829pSilencer2.1-U6-NL1F30For mammalian expression of NL1F30, a dual-specificity aptamer that binds OGT and GFP. Expression is driven by a U6 promoter. This dual-specificity aptamer adopts a flexible linker.
190830pSilencer2.1-U6-NL1F35For mammalian expression of NL1F35, a dual-specificity aptamer that binds OGT and GFP. Expression is driven by a U6 promoter. This dual-specificity aptamer adopts a flexible linker.
190831pSilencer2.1-U6-NL1F50For mammalian expression of NL1F50, a dual-specificity aptamer that binds OGT and GFP. Expression is driven by a U6 promoter. This dual-specificity aptamer adopts a flexible linker.
190832pSilencer2.1-U6-3JB1FFor mammalian expression of 3JB1F, a dual-specificity aptamer that binds OGT and GFP. Expression is driven by a U6 promoter. This dual-specificity aptamer adopts a folded linker.
190833pSilencer2.1-U6-3JB1F+2For mammalian expression of 3JB1F+2, a dual-specificity aptamer that binds OGT and GFP. Expression is driven by a U6 promoter. This dual-specificity aptamer adopts a folded linker.
190834pSilencer2.1-U6-3JB1F+4For mammalian expression of 3JB1F+4, a dual-specificity aptamer that binds OGT and GFP. Expression is driven by a U6 promoter. This dual-specificity aptamer adopts a folded linker.
190835pSilencer2.1-U6-3JB1F+6For mammalian expression of 3JB1F+6, a dual-specificity aptamer that binds OGT and GFP. Expression is driven by a U6 promoter. This dual-specificity aptamer adopts a folded linker.
190836pSilencer2.1-U6-3JB1F+8For mammalian expression of 3JB1F+8, a dual-specificity aptamer that binds OGT and GFP. Expression is driven by a U6 promoter. This dual-specificity aptamer adopts a folded linker.
190837pSilencer2.1-U6-3JB1F+10For mammalian expression of 3JB1F+10, a dual-specificity aptamer that binds OGT and GFP. Expression is driven by a U6 promoter. This dual-specificity aptamer adopts a folded linker.
190838pSilencer2.1-U6-3JB1F+12For mammalian expression of 3JB1F+12, a dual-specificity aptamer that binds OGT and GFP. Expression is driven by a U6 promoter. This dual-specificity aptamer adopts a folded linker.
190839pSilencer2.1-U6-3JB1RFor mammalian expression of 3JB1R, a dual-specificity aptamer that binds OGT and GFP. Expression is driven by a U6 promoter. This dual-specificity aptamer adopts a folded linker.
190840pSilencer2.1-U6-3JB1R+2For mammalian expression of 3JB1R+2, a dual-specificity aptamer that binds OGT and GFP. Expression is driven by a U6 promoter. This dual-specificity aptamer adopts a folded linker.
190841pSilencer2.1-U6-3JB1R+4For mammalian expression of 3JB1R+4, a dual-specificity aptamer that binds OGT and GFP. Expression is driven by a U6 promoter. This dual-specificity aptamer adopts a folded linker.
190842pSilencer2.1-U6-3JB1R+6For mammalian expression of 3JB1R+6, a dual-specificity aptamer that binds OGT and GFP. Expression is driven by a U6 promoter. This dual-specificity aptamer adopts a folded linker.
190843pSilencer2.1-U6-3JB1R+8For mammalian expression of 3JB1R+8, a dual-specificity aptamer that binds OGT and GFP. Expression is driven by a U6 promoter. This dual-specificity aptamer adopts a folded linker.
190844pSilencer2.1-U6-3JB1R+10For mammalian expression of 3JB1R+10, a dual-specificity aptamer that binds OGT and GFP. Expression is driven by a U6 promoter. This dual-specificity aptamer adopts a folded linker.
190845pSilencer2.1-U6-3JB1R+12For mammalian expression of 3JB1R+12, a dual-specificity aptamer that binds OGT and GFP. Expression is driven by a U6 promoter. This dual-specificity aptamer adopts a folded linker.
190846pSilencer2.1-U6-4JC1FFFor mammalian expression of 4JC1FF, a dual-specificity aptamer that binds OGT and GFP. Expression is driven by a U6 promoter. This dual-specificity aptamer adopts a folded linker.
190847pSilencer2.1-U6-4JC1RRFor mammalian expression of 4JC1RR, a dual-specificity aptamer that binds OGT and GFP. Expression is driven by a U6 promoter. This dual-specificity aptamer adopts a folded linker.
190848pSilencer2.1-U6-bc339For mammalian expression of bc339, the RNA aptamer of β-catenin. Expression is driven by a U6 promoter.
190849pSilencer2.1-U6-T1-U6-bc339For mammalian expression of T1 and bc339, the RNA aptamers of ncOGT and β-catenin, respectively. Expression of each aptamer is driven by a U6 promoter.
190850pSilencer2.1-U6-NL8F50For mammalian expression of NL8F50, a dual-specificity aptamer that binds OGT and β-catenin. Expression is driven by a U6 promoter. This dual-specificity aptamer adopts a flexible linker.
190851pSilencer2.1-U6-NL8F70For mammalian expression of NL8F70, a dual-specificity aptamer that binds OGT and β-catenin. Expression is driven by a U6 promoter. This dual-specificity aptamer adopts a flexible linker.
190852pSilencer2.1-U6-NL8F100For mammalian expression of NL8F100, a dual-specificity aptamer that binds OGT and β-catenin. Expression is driven by a U6 promoter. This dual-specificity aptamer adopts a flexible linker.
190853pSilencer2.1-U6-3JB8F+4For mammalian expression of 3JB8F+4, a dual-specificity aptamer that binds OGT and β-catenin. Expression is driven by a U6 promoter. This dual-specificity aptamer adopts a folded linker.
190854pSilencer2.1-U6-3JB8F+12For mammalian expression of 3JB8F+12, a dual-specificity aptamer that binds OGT and β-catenin. Expression is driven by a U6 promoter. This dual-specificity aptamer adopts a folded linker.
190855pSilencer2.1-U6-3JB8R+12For mammalian expression of 3JB8R+12, a dual-specificity aptamer that binds OGT and β-catenin. Expression is driven by a U6 promoter. This dual-specificity aptamer adopts a folded linker.
190856pSilencer2.1-U6-LRS1F50CFor mammalian expression of LRS1F50C, a dual-specificity aptamer that binds OGT and GFP. Expression is driven by a U6 promoter. This dual-specificity aptamer is regulated by riboswitch elements.
190858pcDNA3.1-OGT_optFor mammalian expression of wild-type full-length human ncOGT. The ncOGT is not epitope-tagged, but is codon-optimized for human cells.

Antibodies from Article