The miniature CRISPR-Cas12m effector binds DNA to block transcription.
Wu WY, Mohanraju P, Liao C, Adiego-Perez B, Creutzburg SCA, Makarova KS, Keessen K, Lindeboom TA, Khan TS, Prinsen S, Joosten R, Yan WX, Migur A, Laffeber C, Scott DA, Lebbink JHG, Koonin EV, Beisel CL, van der Oost J
Mol Cell. 2022 Dec 1;82(23):4487-4502.e7. doi: 10.1016/j.molcel.2022.11.003. Epub 2022 Nov 24. PubMed Article
Wu WY, Mohanraju P, Liao C, Adiego-Perez B, Creutzburg SCA, Makarova KS, Keessen K, Lindeboom TA, Khan TS, Prinsen S, Joosten R, Yan WX, Migur A, Laffeber C, Scott DA, Lebbink JHG, Koonin EV, Beisel CL, van der Oost J
Mol Cell. 2022 Dec 1;82(23):4487-4502.e7. doi: 10.1016/j.molcel.2022.11.003. Epub 2022 Nov 24. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 192274 | pCas-Cas12m | Encodes MmCas12m under a constitutive promoter |
| 192275 | pCas-dCas12m | Encodes MmdCas12m (D485A) under a constitutive promoter |
| 192276 | pCas-Cas12m-ΔZF | Encodes MmCas12m ΔZF (H549A, C552A) under a constitutive promoter |
| 192277 | pCas-dCas12m-ΔZF | pCas-dCas12m-ΔZF (D485A, H549A, C552A) under a constitutive promoter |
| 192278 | pCRISPR-RFP entry | Entry vector for cloning MmCas12m spacers. RFP flanked by MmCas12m CRISPR repeats can be digested out with BbsI. |
| 192279 | pTarget-RFP-GFP operon | RFP GFP fusion protein expressed under a constitutive promoter |
| 192280 | pTarget: Ptaq-RFP_PlacIq-GFP | RFP expressed under constitutive promoter Ptaq and GFP expressed under constitutive promoter Placiq |
| 192282 | pCas-pML-1B_6xHis-Cas12m | MmCas12m fused to His-tag for protein purification |
| 192284 | pCas-MmdCas12m-CBE1 | MmdCas12m-CBE1 expressed under a constitutive promoter |