Three Mutations Convert the Selectivity of a Protein Sensor from Nicotinic Agonists to S-Methadone for Use in Cells, Organelles, and Biofluids.
Muthusamy AK, Kim CH, Virgil SC, Knox HJ, Marvin JS, Nichols AL, Cohen BN, Dougherty DA, Looger LL, Lester HA
J Am Chem Soc. 2022 May 18;144(19):8480-8486. doi: 10.1021/jacs.2c02323. Epub 2022 Apr 21.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 220861 | pHHM.X513.iS-methadoneSnFR | Bacterial expression of iS-methadoneSnFR for purification and in vitro assays. |
| 220865 | pTwist-iS-methadoneSnFR-Golgi | Mammalian cell expression for subcellular pharmacokinetics with Golgi targeting of iS-methadoneSnFR |