Systematic discovery of recombinases for efficient integration of large DNA sequences into the human genome.
Durrant MG, Fanton A, Tycko J, Hinks M, Chandrasekaran SS, Perry NT, Schaepe J, Du PP, Lotfy P, Bassik MC, Bintu L, Bhatt AS, Hsu PD
Nat Biotechnol. 2022 Oct 10. pii: 10.1038/s41587-022-01494-w. doi: 10.1038/s41587-022-01494-w. PubMed Article
Durrant MG, Fanton A, Tycko J, Hinks M, Chandrasekaran SS, Perry NT, Schaepe J, Du PP, Lotfy P, Bassik MC, Bintu L, Bhatt AS, Hsu PD
Nat Biotechnol. 2022 Oct 10. pii: 10.1038/s41587-022-01494-w. doi: 10.1038/s41587-022-01494-w. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 193459 | pAF0432 Ef1a-Dn29-2a-EGFP | Expression of Recombinase |
| 193460 | pAF0033 Ef1a-Pa01-2a-EGFP | Expression of Recombinase |
| 193461 | pAF0034 Ef1a-Kp03-2a-EGFP | Expression of Recombinase |
| 193462 | pAF0084 Ef1a-Cp36-2a-EGFP | Expression of Recombinase |
| 193463 | pAF0036 Ef1a-Si74-2a-EGFP | Expression of Recombinase |
| 193464 | pAF0035 Ef1a-Nm60-2a-EGFP | Expression of Recombinase |
| 193465 | pAF0692 Ef1a-Dn29_attD-Puro-p2a-mCherry | Dn29 Donor with promoter for mcherry and puro expression |
| 193466 | pAF0687 Pa01_attP-mCherry donor | Promoterless mcherry donor for Pa01 |
| 193467 | pAF0688 Kp03_attP-mCherry donor | Promoterless mcherry donor for Kp03 |
| 193468 | pAF0185 Cp36_attP-mCherry donor | Promoterless mcherry donor for Cp36 |
| 193469 | pAF0362 Ef1a-Cp36_attP-Puro-p2a-mCherry donor | Cp36 Donor with promoter for mcherry and puro expression |
| 193470 | pAF0451 Si74_attP-mCherry donor | Promoterless mcherry donor for Si74 |
| 193471 | pAF0443 Nm60_attP-mCherry donor | Promoterless mcherry donor for Nm60 |
| 193472 | pAF0689 Ef1a-Pa01_attB-BFP acceptor | Acceptor plasmid for 3 plasmid recombination assay |
| 193473 | pAF0690 Ef1a-Kp03_attB-BFP acceptor | Acceptor plasmid for 3 plasmid recombination assay |
| 193474 | pAF0286 Ef1a-Cp36_attB-BFP acceptor | Acceptor plasmid for 3 plasmid recombination assay |
| 193475 | pAF0474 Ef1a-Si74_attB acceptor | Acceptor plasmid for 3 plasmid recombination assay |
| 193476 | pAF0466 Ef1a-Nm60_attB acceptor | Acceptor plasmid for 3 plasmid recombination assay |