Continuous directed evolution of a compact CjCas9 variant with broad PAM compatibility.
Schmidheini L, Mathis N, Marquart KF, Rothgangl T, Kissling L, Bock D, Chanez C, Wang JP, Jinek M, Schwank G
Nat Chem Biol. 2023 Sep 21. doi: 10.1038/s41589-023-01427-x.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 194059 | p2T-CMV-evoCjCas9-BlastR | Mammalian expression of evoCjCas9 nuclease |
| 194060 | p2T-CMV-evoCjCas9-ABE8e-BlastR | Mammalian expression of evoCjCas9 ABE8e |
| 194061 | p2T-CMV-evoCjCas9-BE4max-BlastR | Mammalian expression of evoCjCas9 CBE4max |
| 194062 | p2T-CMV-evoCjCas9-eAID-BlastR | Mammalian expression of evoCjCas9 eAID |
| 194063 | p2T-CMV-evoCjCas9-PEmax_dRnH-BlastR | Mammalian expression of evoCjCas9 PEmax_deltaRnH |
| 202558 | p2T-CMV-evoCjCas9-TadCDd-BlastR | Mammalian expression of evoCjCas9 TadCDd |
| 202559 | AAV-P3-evoCjCas9-ABE8e_gRNA | P3-evoCjCas9-ABE8e and hU6-BsmBI-gRNA in AAV2 backbone (ITRs) |
| 202561 | AAV-hSyn-evoCjCas9-ABE8e_gRNA | hSyn-evoCjCas9-ABE8e and hU6-BsmBI-gRNA in AAV2 backbone (ITRs) |
| 202562 | AAV-P3-evoCjCas9-eAID_gRNA | P3-evoCjCas9-eAID and hU6-BsmBI-gRNA in AAV2 backbone (ITRs) |
| 202563 | AAV-P3-evoCjCas9-TadCDd_gRNA | P3-evoCjCas9-TadCDd and hU6-BsmBI-gRNA in AAV2 backbone (ITRs) |
| 202564 | LentiGuide_CjCas9-Puro | hU6-BsmBI-gRNA-Puro |