Comprehensive Profiling of Four Base Overhang Ligation Fidelity by T4 DNA Ligase and Application to DNA Assembly.
Potapov V, Ong JL, Kucera RB, Langhorst BW, Bilotti K, Pryor JM, Cantor EJ, Canton B, Knight TF, Evans TC Jr, Lohman GJS
ACS Synth Biol. 2018 Nov 16;7(11):2665-2674. doi: 10.1021/acssynbio.8b00333. Epub 2018 Oct 29.
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Article
These materials are included in the High Complexity Golden Gate Assembly Standards Kit (24 fragment lac cassette).
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 195714 | pGGAselect | pGGAselect is a cloning vector for Golden Gate Assembly using BsaI, BsmBI, and BbsI Type IIS restriction enzymes. |
| 195715 | pUC57-mini v2-HF24_F1 | This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity. |
| 195716 | pUC57-mini v2-HF24_F2 | This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity. |
| 195717 | pUC57-mini v2-HF24_F3 | This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity. |
| 195718 | pUC57-mini v2-HF24_F4 | This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity. |
| 195719 | pUC57-mini v2-HF24_F5 | This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity. |
| 195720 | pUC57-mini v2-HF24_F6 | This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity. |
| 195721 | pUC57-mini v2-HF24_F7 | This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity. |
| 195722 | pUC57-mini v2-HF24_F8 | This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity. |
| 195723 | pUC57-mini v2-HF24_F9 | This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity. |
| 195724 | pUC57-mini v2-HF24_F10 | This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity. |
| 195725 | pUC57-mini v2-HF24_F11 | This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity. |
| 195726 | pUC57-mini v2-HF24_F12 | This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity. |
| 195727 | pUC57-mini v2-HF24_F13 | This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity. |
| 195728 | pUC57-mini v2-HF24_F14 | This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity. |
| 195729 | pUC57-mini v2-HF24_F15 | This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity. |
| 195730 | pUC57-mini v2-HF24_F16 | This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity. |
| 195731 | pUC57-mini v2-HF24_F17 | This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity. |
| 195732 | pUC57-mini v2-HF24_F18 | This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity. |
| 195733 | pUC57-mini v2-HF24_F19 | This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity. |
| 195734 | pUC57-mini v2-HF24_F20 | This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity. |
| 195735 | pUC57-mini v2-HF24_F21 | This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity. |
| 195736 | pUC57-mini v2-HF24_F22 | This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity. |
| 195737 | pUC57-mini v2-HF24_F23 | This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity. |
| 195738 | pUC57-mini v2-HF24_F24 | This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity. |