Generating a New sgRNA Vector, pGL3-U6-sgRNA-PGK-mRFP-T2A-PuroR, to Improve Base Editing.
Ayo TE, Xu H
J Genome Ed Regul. 2022;2:246146. doi: 10.32371/jger/246146. Epub 2022 Dec 17.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 194925 | pGL3-U6-sgRNA-PGK- mRFP-T2A-PuroR | mRFP and T2A linker are inserted in between the hPGK promoter and the puromycin resistance gene (PuroR) on pGL3-U6-sgRNA-PGK-puromycin to allow simultaneous monitoring and enrichment of transfected ho |