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Discovery and characterization of novel type I-D CRISPR-guided transposons identified among diverse Tn7-like elements in cyanobacteria.
Hsieh SC, Peters JE
Nucleic Acids Res. 2023 Jan 25;51(2):765-782. doi: 10.1093/nar/gkac1216. PubMed Article

Plasmids from Article

ID Plasmid Purpose
195296pOPO640pTA106-Myacmini-KanR, donor plasmid with mini-transposon of McCAST (Tn7575).
195297pOPO635pACYC-plac-MycaTnsABC, lac promoter expressing transposase genes TnsABC of McCAST (Tn7575).
195299pOPO636pACYC-plac-MycaTnsABCQ, lac promoter expressing transposase genes TnsABCQ of McCAST (Tn7575).
195300pOPO685pBAD322-MycaIDCas-PaqCI, arapBAD promoter expressing type I-D Cascade and one spacer array of McCAST (Tn7575), the single spacer is replaced with PaqCI entry site.
195302pOPO717pBAD322-MycaIDCas-lacZsp5, arapBAD promoter expressing type I-D Cascade and one spacer array of McCAST (Tn7575), the single spacer is lacZ spacer 5.
195303pOPO794pBAD322-MycaIDCas-ΔCas6-RGR-PaqCI, arapBAD promoter expressing type I-D Cascade of McCAST (Tn7575) without cas6 gene and ribozyme flanked PaqCI entry site.
195306pOPO812pBAD322-MycaIDCas-ΔCas6-RGR-lacZs, arapBAD promoter expressing type I-D Cascade of McCAST (Tn7575) without cas6 gene and ribozyme flanked lacZ spacer 5.
195307pOPO646pBAD322-MycaTnsD, arapBAD promoter expressing cyanobacterial tRNA-Leu gene targeting TnsD protein of McCAST (Tn7575).

Antibodies from Article