Biochemical and biophysical characterization of the nucleic acid binding properties of the RNA/DNA binding protein EWS.
Selig EE, Bhura R, White MR, Akula S, Hoffman RD, Tovar CN, Xu X, Booth RE, Libich DS
Biopolymers. 2023 May;114(5):e23536. doi: 10.1002/bip.23536. Epub 2023 Mar 17.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 188045 | pAG8Ha-EWS_RRM(359-447) | Expresses His8-EWS_RRM(359-447) with a TEV site for removal of the His8 tag. |
| 188046 | pAG8Ha-EWS_RRM-RGG(359-513) | Expresses His8-EWS_RRM-RGG(359-513) with a TEV site for removal of the His8 tag. |
| 195868 | pAG8Ha-His-EWSR1-359-549 | Expresses the RRM-RG2-ZnF (359-549) of EWS |
| 199439 | pEmal10H-MBP-EWSR1-449-513 | Expresses RG2 (the second RG-rich region, 449-513) of EWS |