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The Axin scaffold protects the kinase GSK3beta from cross-pathway inhibition.
Gavagan M, Jameson N, Zalatan JG
Elife. 2023 Aug 7;12:e85444. doi: 10.7554/eLife.85444. PubMed Article

Plasmids from Article

ID Plasmid Purpose
196182pMG024 MBP-GSK3β-HA-His, GST_λPPaseCo-expresses MBP-GSK3β-HA-His (human GSK3β as a fusion protein with MBP, HA, and His-tags) and GST_λPPase in E.coli to produce unphosphorylated MBP-GSK3β-HA-His
196184pES002 MBP-GSK3β_S9A-HA-HisExpresses MBP-GSK3β_S9A-HA-His (human Glycogen Synthase Kinase 3β S9A mutant as a fusion protein with MBP, HA, and His- tags) in E.coli
196185pMG071 MBP-GSK3β_S9A-HA-His, GST_λPPaseCo-expresses MBP-GSK3β_S9A-HA-His (human GSK3β with S9A mutation as a fusion protein with MBP, HA, and His-tags) and GST_λPPase in E.coli to produce unphosphorylated MBP-GSK3β_S9A-HA-His

Antibodies from Article