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pMG071 MBP-GSK3β_S9A-HA-His, GST_λPPase
(Plasmid #196185)


Item Catalog # Description Quantity Price (USD)
Plasmid 196185 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
    New England Biolabs
  • Backbone size w/o insert (bp) 6716
  • Total vector size (bp) 9466
  • Modifications to backbone
    Modified to include an N-terminal TEV-cleavable MBP tag and a C-terminal His6 tag
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    Low Copy

Gene/Insert 1

  • Gene/Insert name
  • Alt name
    Glycogen Synthase Kinase 3β
  • Species
    H. sapiens (human)
  • Insert Size (bp)
  • Mutation
    changed Serine 9 to Alanine
  • GenBank ID
    2932 BC000251
  • Entrez Gene
  • Promoter Tac
  • Tags / Fusion Proteins
    • MBP (N terminal on backbone)
    • His6 (C terminal on backbone)
    • HA (C terminal on insert)

Cloning Information for Gene/Insert 1

  • Cloning method Gibson Cloning
  • 5′ sequencing primer MalE Forward GGTCGTCAGACTGTCGATGAAGCC
  • 3′ sequencing primer GST_N Reverse GAGTGGGTTGCACAAG
  • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
  • Alt name
    Lambda Phosphatase
  • Alt name
    serine/threonine protein phosphatase [Escherichia phage Lambda]
  • Species
    Bacteriophage lambda
  • Insert Size (bp)
  • GenBank ID
  • Promoter Tac
  • Tag / Fusion Protein
    • GST (C terminal on insert)

Cloning Information for Gene/Insert 2

  • Cloning method Gibson Cloning
  • 5′ sequencing primer pGEX 5' GGGCTGGCAAGCCACGTTTGGTG
  • 3′ sequencing primer M13F Reverse TGTAAAACGACGGCCAGT
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Addgene #14753

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMG071 MBP-GSK3β_S9A-HA-His, GST_λPPase was a gift from Jesse Zalatan (Addgene plasmid # 196185 ; ; RRID:Addgene_196185)
  • For your References section:

    The Axin scaffold protects the kinase GSK3beta from cross-pathway inhibition. Gavagan M, Jameson N, Zalatan JG. Elife. 2023 Aug 7;12:e85444. doi: 10.7554/eLife.85444. 10.7554/eLife.85444 PubMed 37548359