Overexpression of the WWE domain of RNF146 modulates poly-(ADP)-ribose dynamics at sites of DNA damage.
Al-Rahahleh RQ, Roos WP, Saville KM, Andrews JF, Wu Z, Koczor CA, Prakash A, Sobol RW
DNA Repair (Amst). 2025 Jun;150:103845. doi: 10.1016/j.dnarep.2025.103845. Epub 2025 May 21.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 196228 | pLV-Hygro-EF1A-RNF146(92-168)-EGFP | EGFP fused to the C-terminus of a WWE domain within RNF146 & a hygromycin resistance cassette |
| 196229 | pLV-Hygro-EF1A-TRIP12(749-836)-EGFP | EGFP fused to the C-terminus of a WWE domain within TRIP12 & a hygromycin resistance cassette |
| 196230 | pLV-Hygro-EF1A-DTX2(8-97)-EGFP | EGFP fused to the C-terminus of first WWE domain within Deltex2 & a hygromycin resistance cassette |
| 196232 | pLV-Hygro-EF1A-DTX2(8-174)-EGFP | EGFP fused to the C-terminus of tandem WWE domains within Deltex2 & a hygromycin resistance cassette |
| 196233 | pLV-Hygro-EF1A-Af1521(WT)-EGFP | EGFP fused to the C-terminus of Af1521 (WT) macrodomain & a hygromycin resistance cassette |
| 196235 | pLV-Hygro-EF1A-Af1521(K35E/Y145R)-EGFP | EGFP fused to the C-terminus of Af1521(K35E/Y145R) macrodomain & a hygromycin resistance cassette |
| 196236 | pLV-Hygro-EF1A-RNF146(100-182)-myc | RNF146(100-182) WWE domain with a myc tag fused to the C terminus & a hygromycin resistance cassette |
| 196237 | pLV-Hygro-EF1A-Af1521(K35E/Y145R)-myc | Af1521(K35E/Y145R) macrodomain with a myc tag fused to the C terminus & a hygromycin resistance cassette |
| 196238 | pLV-Hygro-EF1A-Af1521(G42E)-myc | Af1521(G42E) macrodomain with a myc tag fused to the C terminus & a hygromycin resistance cassette |
| 196239 | pGEX-4T-3-GST-Tev-Af1521-myc | N-terminal GST fusion of Af1521(WT) with a TEV protease site located between the GST tag and Af1521 and a myc tag on the C terminus |
| 196240 | pGEX-4T-3-GST-Tev-RNF146(100-182)-myc | N-terminal GST fusion of RNF146(100-182) WWE domain with a TEV protease site located between the GST tag and Af1521 and a myc tag on the C terminus |
| 196241 | pGEX-4T-3-GST-Tev-Af1521(K35E/Y145R)-myc | N-terminal GST fusion of Af1521(K35E/Y145R) with a TEV protease site located between the GST tag and Af1521 and a myc tag on the C terminus |
| 238091 | pLV-EF1A-RNF146-45-182-EGFP-Hygro | Expression of structurally predicted WWE domain of RNF146 with EGFP on C terminal |
| 238092 | pLV-EF1A-TRIP12-391-938-EGFP-Hygro | Expression of structurally predicted WWE domain of TRIP12 with EGFP on C terminal |
| 238093 | pLV-EF1A-DTX1-1-175-EGFP-Hygro | Expression of structurally predicted WWE domain of DTX1 with EGFP on C terminal |
| 238094 | pLV-EF1A-DTX2-1-178-EGFP-Hygro | Expression of structurally predicted WWE domain of DTX2 with EGFP on C terminal |
| 238095 | pLV-EF1A-DTX4-1-166-EGFP-Hygro | Expression of structurally predicted WWE domain of DTX4 with EGFP on C terminal |
| 238096 | pLV-EF1A-HUWE1-1503-1780_EGFP-Hygro | Expression of structurally predicted WWE domain of HUWE1 with EGFP on C terminal |
| 238097 | pLV-EF1A-PARP11-22-122-EGFP-Hygro | Expression of structurally predicted WWE domain of PARP11 with EGFP on C terminal |
| 238098 | pLV-EF1A-PARP12-298-462-Hygro | Expression of structurally predicted WWE domain of PARP12 with EGFP on C terminal |
| 238099 | pLV-EF1A-PARPT-334-406-Hygro | Expression of structurally predicted WWE domain of PARPT with EGFP on C terminal |
| 238100 | pLV-EF1A-PARP14-1535-1601-Hygro | Expression of structurally predicted WWE domain of PARP14 with EGFP on C terminal |
| 238101 | pLV-EF1A-DDHD2-30-202-Hygro | Expression of structurally predicted WWE domain of DDHD2 with EGFP on C terminal |
| 238102 | pLV-EF1A-ZCCHV-594-684-Hygro | Expression of structurally predicted WWE domain of ZCCHV with EGFP on C terminal |