Heritable transgene-free genome editing in plants by grafting of wild-type shoots to transgenic donor rootstocks.
Yang L, Machin F, Wang S, Saplaoura E, Kragler F
Nat Biotechnol. 2023 Jan 2. doi: 10.1038/s41587-022-01585-8. PubMed Article
Yang L, Machin F, Wang S, Saplaoura E, Kragler F
Nat Biotechnol. 2023 Jan 2. doi: 10.1038/s41587-022-01585-8. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 196977 | Cas9 TLS1 | Expresses Cas9 fused with TLS1 mobility signal that confers graft mobility. Induced by estradiol. |
| 196978 | Cas9 TLS2 | Expresses Cas9 fused with TLS2 mobility signal that confers graft mobility. Induced by estradiol. |
| 196980 | gRNA NIA TLS1 | Expresses two gRNAs targeting Arabidopsis NIA1 gene for knockout. Each gRNA is fused to TLS1 mobility signal that confers graft mobility. |
| 196981 | gRNA NIA TLS2 | Expresses two gRNAs targeting Arabidopsis NIA1 gene for knockout. Each gRNA is fused to TLS2 mobility signal that confers graft mobility. |
| 196983 | Fragment1 with TLS2 | Gateway-compatible gRNA backbone with TLS2 mobility signal. Template for the addition of target sequences to produce 2x graft-mobile gRNA-TLS2. |
| 198727 | Cas9 Null | Expresses Cas9 without a mobility signal and the mRNA is not graft mobile. Induced by estradiol. |
| 198728 | gRNA NIA Null | Expresses two gRNAs targeting Arabidopsis NIA1 gene for knockout. Neither gRNA is fused to a mobility signal and are therefore non-mobile. |
| 198729 | Fragment 1 Null | Gateway-compatible gRNA backbone . Template for the addition of target sequences to produce 2x gRNA. |