Unmyelinated sensory neurons use Neuregulin signals to promote myelination of interneurons in the CNS.
Lysko DE, Talbot WS
Cell Rep. 2022 Nov 15;41(7):111669. doi: 10.1016/j.celrep.2022.111669.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 197647 | zCREST3:Cas9green; T2_gRNA | pDEL160; transgenic construct to express cell-specific Cas9 in sensory neurons; ubiquitous nrg1 type II sgRNA |
| 199332 | zCREST3:Cas9green; T1_gRNA | pDEL159; transgenic construct to express cell-specific Cas9 in sensory neurons; ubiquitous nrg1 type I sgRNA |
| 199333 | zCREST3:Cas9green; EGF_gRNA | pDEL158; transgenic construct to express cell-specific Cas9 in sensory neurons; ubiquitous nrg1 EGF sgRNA |
| 199334 | zCREST3:redCas9; control_gRNA | pDEL157; transgenic construct to express cell-specific Cas9 in sensory neurons; ubiquitous control sgRNA |
| 199335 | zCREST3:Cas9green; T3_gRNA | pDEL161; transgenic construct to express cell-specific Cas9 in sensory neurons; ubiquitous nrg1 type III sgRNA |
| 199336 | pTol2_claudink:Cas9green; control_gRNA | pDEL143; transgenic construct to express cell-specific Cas9 in oligodendrocytes; ubiquitous control sgRNA |
| 199337 | pTol2_NBT:Cas9green; erbb2_gRNA171 | pDEL134; transgenic construct to express cell-specific Cas9 in neurons; ubiquitous erbb2 sgRNA |
| 199338 | pTol2_LSEC:Cas9green; erbb2_gRNA171 | pDEL135; transgenic construct to express cell-specific Cas9 in sensory neurons; ubiquitous erbb2 sgRNA |
| 199339 | pTol2_claudink:Cas9green; erbb2_gRNA171 | pDEL142; transgenic construct to express cell-specific Cas9 in oligodendrocytes; ubiquitous erbb2 sgRNA |
| 199341 | p3E_afp_UTR | pAMS448; Gateway 3' entry clone encoding ocean pout antifreeze (afp) protein 3'UTR |
| 199385 | pDest_U6_A2_CG2_erbb2_gRNA171 | pDEL133; Gateway Destination vector for cell-type specific CRISPR encoding erbb2 sgRNA |
| 199386 | pDest_U6_A2_CG2_typeEGF_gRNA | pDEL110; Gateway Destination vector for cell-type specific CRISPR encoding nrg1 EGF sgRNA |
| 199387 | pDest_U6_A2_CG2_T1_gRNA | pDEL111; Gateway Destination vector for cell-type specific CRISPR encoding nrg1 type I sgRNA |
| 199388 | pDest_U6_A2_CG2_T2_gRNA | pDEL112; Gateway Destination vector for cell-type specific CRISPR encoding nrg1 type II sgRNA |
| 199389 | pDest_U6_A2_CG2_T3_gRNA | pDEL113; Gateway Destination vector for cell-type specific CRISPR encoding nrg1 type III sgRNA |
| 199393 | p5E_(-3kb)nrg1 type II | pDEL61; Gateway 5' Entry clone encoding nrg1 type II upstream regulatory sequence |
| 199394 | pTol2_(-3kb)nrg1_typeII:GFP-CAAX | pDEL63; transgenic construct to express GFP-CAAX under nrg1 type II regulatory region control |
| 199395 | p5E_ngn1_LSE_C | pDEL98; Gateway 5' Entry clone encoding select ngn1 upstream regulatory sequence |