A cAMP Sensor Based on Ligand-Dependent Protein Stabilization.
Sidoli M, Chen LC, Lu AJ, Wandless TJ, Talbot WS
ACS Chem Biol. 2022 Aug 19;17(8):2024-2030. doi: 10.1021/acschembio.2c00333. Epub 2022 Jul 15.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 241360 | pTol2_Ubi_N41 CNBD AcGFP | pMPS37; ubiquitously expresses cAMP sensor; zebrafish transgene |
| 241361 | pTol2_Ubi_N41_R307Q CNBD AcGFP | pMPS55; ubiquitously expresses cAMP sensor(cAMP-insensitive mutant control); zebrafish transgene |
| 241362 | pME_N41_CNBD_AcGFP | pMPS15; middle entry clone holding N41_acgfp_cAMP sensor |
| 241363 | pME_N41_R307Q_CNBD_AcGFP | pMPS53; middle entry clone holding N41_R307Q_CNBD_acgfp (cAMP-insensitive mutant control sensor) |