Improving cell-free glycoprotein synthesis by characterizing and enriching native membrane vesicles.
Hershewe JM, Warfel KF, Iyer SM, Peruzzi JA, Sullivan CJ, Roth EW, DeLisa MP, Kamat NP, Jewett MC
Nat Commun. 2021 Apr 22;12(1):2363. doi: 10.1038/s41467-021-22329-3. PubMed Article
Hershewe JM, Warfel KF, Iyer SM, Peruzzi JA, Sullivan CJ, Roth EW, DeLisa MP, Kamat NP, Jewett MC
Nat Commun. 2021 Apr 22;12(1):2363. doi: 10.1038/s41467-021-22329-3. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 198204 | pSF-NgPglO | pSN18 derivative encoding N. gonorrhoeae PglO with C-terminal FLAG epitope tag |
| 198210 | pJL1-sfGFP-DQNAT | pJL1 plasmid encoding superfolder GFP modified at the C-terminus with 30 amino acids containing an optimal DQNAT sequon followed by a 6x-His tag |
| 198211 | pJL1-sfGFP-AQNAT | pJL1 plasmid encoding superfolder GFP modified at the C-terminus with 30 amino acids containing a non-permissible AQNAT sequon followed by a 6x-His tag |
| 198212 | pJL1-sfGFP-MOOR | pJL1 plasmid encoding superfolder GFP modified at the C-terminus with 32 amino acids containing the minimum optimal O-linked recognition site (MOOR) followed by a 6x-His tag |
| 198213 | pJL1-sfGFP-MOORmut | pJL1 plasmid encoding superfolder GFP modified at the C-terminus with 32 amino acids containing a non-permissible minimum optimal O-linked recognition site (MOORmut) followed by a 6x-His tag |
| 198214 | pJL1-AcrA | pJL1 plasmid encoding wildtype AcrA from C. jejuni containing 2 native internal glycosylation sites recognized by PglB and a C-terminal 6x-His tag |
| 198215 | pJL1-MBP-DQNAT | pJL1 plasmid encoding E. coli maltose binding protein (MBP) modified at the C-terminus with 30 amino acids containing one optimal DQNAT sequon followed by a 6x-His tag |
| 198216 | pJL1-PD-DQNAT | pJL1 plasmid encoding H. influenzae protein D (PD) modified at the C-terminus with 30 amino acids containing one optimal DQNAT sequon followed by a 6x-His tag |
| 199305 | pSF-CjPglB | pSN18 derivative encoding C.jejuni PglB with C-terminal FLAG epitope tag |