A toolkit for recombinant production of seven human EGF family growth factors in active conformation.
Ferreira AS, Lopacinski A, Batista M, Hiraiwa PM, Guimaraes BG, Zanchin NIT
Sci Rep. 2022 Mar 23;12(1):5034. doi: 10.1038/s41598-022-09060-9. PubMed Article
Ferreira AS, Lopacinski A, Batista M, Hiraiwa PM, Guimaraes BG, Zanchin NIT
Sci Rep. 2022 Mar 23;12(1):5034. doi: 10.1038/s41598-022-09060-9. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 199229 | pET32a-hAREG | Bacterial production of soluble, active target proteins; Nterm thrombin and enterokinase cleavage sites. |
| 199230 | pET32a-hBTC | Bacterial production of soluble, active target proteins; Nterm thrombin and enterokinase cleavage sites. |
| 199231 | pET32a-hEGF | Bacterial production of soluble, active target proteins; Nterm thrombin and enterokinase cleavage sites. |
| 199232 | pET32a-hEPGN | Bacterial production of soluble, active target proteins; Nterm thrombin and enterokinase cleavage sites. |
| 199233 | pET32a-hEPR | Bacterial production of soluble, active target proteins; Nterm thrombin and enterokinase cleavage sites. |
| 199234 | pET32a-hHBEGF | Bacterial production of soluble, active target proteins; Nterm thrombin and enterokinase cleavage sites. |
| 199236 | pET32a-hTGFa | Bacterial production of soluble, active target proteins; Nterm thrombin and enterokinase cleavage sites. |