pET32a-hTGFa
(Plasmid #199236)

• Purpose: Bacterial production of soluble, active target proteins; Nterm thrombin and enterokinase cleavage sites.
• Depositing Lab: Nilson Zanchin
• Publication: Ferreira et al Sci Rep. 2022 Mar 23;12(1):5034. doi: 10.1038/s41598-022-09060-9.

Backbone

• Vector backbone: pET32a(+)
• Backbone manufacturer: EMD Biosciences
• Backbone size w/o insert (bp): 5863
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: Transforming growth factor alpha, partial
• Alt name: TFGA
• Species: H. sapiens (human)
• Insert Size (bp): 150
• Entrez Gene: TGFA (a.k.a. TFGA)
• Promoter: T7
• Tag / Fusion Protein:
  • TrxA-6xHis-S-tag (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Unknown (unknown if destroyed)
• 3′ cloning site: Unknown (unknown if destroyed)
• 5′ sequencing primer: T7 promoter
• 3′ sequencing primer: T7 terminator

Resource Information

• Supplemental Documents:
  • pET32a-hTGFa.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry