CRIMP: A CRISPR/Cas9 Insertional Mutagenesis Protocol and the CRIMP Toolkit
Miles LB, Calcinotto V, Sonntag C, Lee C, Bryson-Richardson RJ
bioRxiv 2023 Article
Miles LB, Calcinotto V, Sonntag C, Lee C, Bryson-Richardson RJ
bioRxiv 2023 Article
These plasmids are available to order all together as the CRIMPkit.
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 199469 | pSA_0_mKate2_synCoTC | Site directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTC |
| 199470 | pSA_1_mKate2_synCoTC | Site directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTC |
| 199471 | pSA_2_mKate2_synCoTC | Site directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTC |
| 199472 | pSA_0_mTagBFP2_synCoTC | Site directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTC |
| 199473 | pSA_1_mTagBFP2_synCoTC | Site directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTC |
| 199474 | pSA_2_mTagBFP2_synCoTC | Site directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTC |
| 199475 | pSA_0_mTagBFP2-T2A-sGFP1-10_synCoTC | Site directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTC |
| 199476 | pSA_1_mTagBFP2-T2A-sGFP1-10_synCoTC | Site directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTC |
| 199477 | pSA_2_mTagBFP2-T2A-sGFP1-10_synCoTC | Site directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTC |
| 199478 | pSA_0_mTagBFP2-T2A-sGFP11x7_synCoTC | Site directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTC |
| 199479 | pSA_1_mTagBFP2-T2A-sGFP11x7_synCoTC | Site directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTC |
| 199480 | pSA_2_mTagBFP2-T2A-sGFP11x7_synCoTC | Site directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTC |
| 199481 | pSA_0_T2A-Gal4vp16_synCoTC_4xnrUAS-mKate2 | Site directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTC |
| 199482 | pSA_1_T2A-Gal4vp16_synCoTC_4xnrUAS-mKate2 | Site directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTC |
| 199483 | pSA_2_T2A-Gal4vp16_synCoTC_4xnrUAS-mKate2 | Site directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTC |
| 199484 | pSA_0_T2A-Gal4vp16_synCoTC_4xnrUAS-mTagBFP2 | Site directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTC |
| 199485 | pSA_1_T2A-Gal4vp16_synCoTC_4xnrUAS-mTagBFP2 | Site directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTC |
| 199486 | pSA_2_T2A-Gal4vp16_synCoTC_4xnrUAS-mTagBFP2 | Site directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTC |
| 199487 | pSA_0_T2A-Gal4vp16_synCoTC_4xnrUAS-mTagBFP2-T2A-sGFP1-10 | Site directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTC |
| 199488 | pSA_1_T2A-Gal4vp16_synCoTC_4xnrUAS-mTagBFP2-T2A-sGFP1-10 | Site directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTC |
| 199489 | pSA_2_T2A-Gal4vp16_synCoTC_4xnrUAS-mTagBFP2-T2A-sGFP1-10 | Site directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTC |
| 199490 | pSA_0_T2A-Gal4vp16_synCoTC_4xnrUAS-mTagBFP2-T2A-sGFP11x7 | Site directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTC |
| 199491 | pSA_1_T2A-Gal4vp16_synCoTC_4xnrUAS-mTagBFP2-T2A-sGFP11x7 | Site directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTC |
| 199492 | pSA_2_T2A-Gal4vp16_synCoTC_4xnrUAS-mTagBFP2-T2A-sGFP11x7 | Site directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTC |
| 199493 | pKozak_mTagBFP2_synCoTC | Site directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTC |
| 199494 | pKozak_mTagBFP2-T2A-sGFP1-10_synCoTC | Site directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTC |
| 199495 | pKozak_mTagBFP2-T2A-sGFP11x7_synCoTC | Site directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTC |
| 199496 | pKozak_Gal4vp16_synCoTC_4xnrUAS-mTagBFP2 | Site directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTC |
| 199497 | pKozak_Gal4vp16_synCoTC_4xnrUAS-mTagBFP2-T2A-sGFP1-10 | Site directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTC |
| 199498 | pKozak_Gal4vp16_synCoTC_4xnrUAS-mTagBFP2-T2A-sGFP11x7 | Site directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTC |