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High-content image-based pooled screens reveal regulators of synaptogenesis.

Le A, Biederer T, Blainey PC
Cell Rep. 2025 Jun 24;44(7):115889. doi: 10.1016/j.celrep.2025.115889. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
200167pLV-SV40-puro-EF1a-HA-NLGN1-bcExpresses HA-tagged human NLGN1
220436pFSW-BsmBI-shRNA-hSyn-GFPLentiviral shRNA cloning backbone with GFP
220437pLV-SV40-puro-EF1a-HA-NLGN1dAChE-bcExpresses HA-tagged human NLGN1 with K578A/V579A substitutions

Antibodies from Article