Mammalian Genomic Manipulation with Orthogonal Bxb1 DNA Recombinase Sites for the Functional Characterization of Protein Variants.
Roelle SM, Kamath ND, Matreyek KA
ACS Synth Biol. 2023 Nov 3. doi: 10.1021/acssynbio.3c00355. PubMed Article
Roelle SM, Kamath ND, Matreyek KA
ACS Synth Biol. 2023 Nov 3. doi: 10.1021/acssynbio.3c00355. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 200630 | G384A_pLenti-TetBattP(GT)-BFP-2A-iCasp9-2A-Blast_rtTA3 | Negative selection blasticidin resistant Tet-inducible Matreyek Bxb1(GT) landing pad |
| 200631 | G417A_pLenti-Tet-BattP(GA)-BFP-2A-iCasp9-2A-Blast_rtTA3 | Negative selection blasticidin resistant Tet-inducible Matreyek Bxb1(GA) landing pad |
| 200632 | G413A_BattB(GA)-EGFP | Strong EGFP fluorescence recombination plasmid for Matreyek Bxb1(GA) landing pad |
| 200633 | G421C_BattB(GA)_mCherry | Strong mCherry fluorescence recombination plasmid for Matreyek Bxb1(GA) landing pad |
| 200634 | G718A_BattB(GA)_TKterm_AttB(GT)_sGFP-PTEN-IRES-mCherry-P2A-HygroR_noTerm_attP(GA)_IRES-UnaG-2A-Puro-Term | Plasmid self-excising recombination plasmid for Matreyek Bxb1(GT) landing pad |
| 200635 | G783A_pLenti-Tet-BattP(GA)-miRFP670-2A-iCasp9-2A-Hygro_rtTA3 | Negative selection hygromycin resistant Tet-inducible Matreyek Bxb1(GA) landing pad for dual landing pad cells |
| 200636 | G1052C_BattB(GA)_EGFP_IRES-Spycatcher-sfmCherry3C(1-10)-P2A-shBleR | Strong EGFP fluorescence recombination plasmid for Matreyek Bxb1(GA) landing pad in dual landing pad cells |
| 200637 | J135F_BattB_Vif(T27X4)-IRES-SpyTag-sfmCherry2(11)-PuroR | Recombination plasmid for Matreyek Bxb1(GT) landing pad in dual landing pad cells |
| 200638 | G1095C_BattB(GA)_jGCaMP7c_IRES_shBleR-P2A-HygroR | jGCaMP7c recombination plasmid for Matreyek Bxb1(GA) landing pad in dual landing pad cells |
| 200639 | pNK005C_BattB_mkozak_STIM1_IRES_mCherry-H2A-P2A-PuroR | Low STIM1 abundance recombination plasmid for Matreyek Bxb1(GT) landing pad in dual landing pad cells |