Universal loop assembly: open, efficient and cross-kingdom DNA fabrication.
Pollak B, Matute T, Nunez I, Cerda A, Lopez C, Vargas V, Kan A, Bielinski V, von Dassow P, Dupont CL, Federici F
Synth Biol (Oxf). 2020;5(1):ysaa001. doi: 10.1093/synbio/ysaa001. Epub 2020 Feb 5. PubMed Article
Pollak B, Matute T, Nunez I, Cerda A, Lopez C, Vargas V, Kan A, Bielinski V, von Dassow P, Dupont CL, Federici F
Synth Biol (Oxf). 2020;5(1):ysaa001. doi: 10.1093/synbio/ysaa001. Epub 2020 Feb 5. PubMed Article
These materials are included in the Diatom uLoop Assembly Kit.
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 202091 | pCA_o-1 | Receiver cloning vector for odd-1 level constructs. Contains lacZ for blue/white screening. |
| 202092 | pCA_o-2 | Receiver cloning vector for odd-2 level constructs. Contains lacZ for blue/white screening. |
| 202093 | pCA_o-3 | Receiver cloning vector for odd-3 level constructs. Contains lacZ for blue/white screening. |
| 202094 | pCA_o-4 | Receiver cloning vector for odd-4 level constructs. Contains lacZ for blue/white screening. |
| 202095 | pL0R_lacZ | Receiver cloning vector for making level 0 parts. Contains lacZ for blue/white screening. |
| 202096 | pCA_e-1 | Receiver cloning vector for even-1 level constructs. Contains lacZ for blue/white screening. |
| 202097 | pCA_e-2 | Receiver cloning vector for even-2 level constructs. Contains lacZ for blue/white screening. |
| 202098 | pCA_e-3 | Receiver cloning vector for even-3 level constructs. Contains lacZ for blue/white screening. |
| 202099 | pCA_e-4 | Receiver cloning vector for even-4 level constructs. Contains lacZ for blue/white screening. |
| 202100 | pL0_AB-H4 | Level 0 plasmid containing the promoter from gene 34971 from Phaeodactylum tricornutum with AB overhangs used to build a level 1 construct. |
| 202101 | pL0_AB-NR | Level 0 plasmid containing the promoter from gene 54983 from Phaeodactylum tricornutum with AB overhangs used to build a level 1 construct. |
| 202102 | pL0_AB-P49 | Level 0 plasmid containing the promoter from gene 49202 from Phaeodactylum tricornutum with AB overhangs used to build a level 1 construct. |
| 202103 | pL0_AB-OEE3 | Level 0 plasmid containing the promoter from gene 54499 from Phaeodactylum tricornutum with AB overhangs used to build a level 1 construct. |
| 202104 | pL0_AC-epyC | Level 0 plasmid containing the promoter from gene 41316 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct. |
| 202105 | pL0_AC-BCA | Level 0 plasmid containing the promoter from gene 51305 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct. |
| 202106 | pL0_AC-47740 | Level 0 plasmid containing the promoter from gene 47740 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct. |
| 202107 | pL0_AC-GEF | Level 0 plasmid containing the promoter from gene 41365 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct. |
| 202108 | pL0_AC-51183 | Level 0 plasmid containing the promoter from gene 51183 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct. |
| 202109 | pL0_AC-54730 | Level 0 plasmid containing the promoter from gene 54730 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct. |
| 202110 | pL0_AC-27877 | Level 0 plasmid containing the promoter from gene 27877 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct. |
| 202111 | pL0_AC-fbaC5 | Level 0 plasmid containing the promoter from gene 41423 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct. |
| 202112 | pL0_AC-CaMK2 | Level 0 plasmid containing the promoter from gene 39236 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct. |
| 202113 | pL0_AC-act1 | Level 0 plasmid containing the promoter from gene 29812 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct. |
| 202114 | pL0_AC-sit4 | Level 0 plasmid containing the promoter from gene 48707 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct. |
| 202115 | pL0_AC-NRT | Level 0 plasmid containing the promoter from gene 54101 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct. |
| 202116 | pL0_AC-HO | Level 0 plasmid containing the promoter from gene 5902 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct. |
| 202117 | pL0_AC-lysA | Level 0 plasmid containing the promoter from gene 21592 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct. |
| 202118 | pL0_AC-vapol | Level 0 plasmid containing the promoter from gene 47434 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct. |
| 202119 | pL0_AC-fcpF | Level 0 plasmid containing the promoter from gene 51230 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct. |
| 202120 | pL0_AC-P49 | Level 0 plasmid containing the promoter from gene 49202 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct. |
| 202121 | pL0_AC-fcpB | Level 0 plasmid containing the promoter from gene 25172 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct. |
| 202122 | pL0_AC-H4 | Level 0 plasmid containing the promoter from gene 34971 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct. |
| 202123 | pL0_AC-NR | Level 0 plasmid containing the promoter from gene 54983 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct. |
| 202124 | pL0_AC-GPI1 | Level 0 plasmid containing the promoter from gene 23924 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct. |
| 202125 | pL0_AC-Fld | Level 0 plasmid containing the promoter from gene 23658 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct. |
| 202126 | pL0_AC-dph1 | Level 0 plasmid containing the promoter from gene 54330 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct. |
| 202127 | pL0_AC-glna | Level 0 plasmid containing the promoter from gene 22357 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct. |
| 202128 | pL0_AC-acc2 | Level 0 plasmid containing the promoter from gene 55209 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct. |
| 202129 | pL0_AC-CEN_ARS_HIS | Level 0 plasmid containing a centromere sequence that is recognized in diatoms with AC overhangs used to build level 1 constructs. |
| 202130 | pL0_AF-spacer | Level 0 plasmid containing a small non-coding spacer sequence with AF overhangs used to build level 1 constructs. |
| 202131 | pL0_BC-chl_sig_pep | Level 0 plasmid containing a kozak consensus sequence and a chloroplast signal peptide sequence with BC overhangs used to build level 1 constructs. |
| 202132 | pL0_BC-pyr_comp_sig_pep | Level 0 plasmid containing a kozak consensus sequence and a pyrenoid compartment signal peptide sequence with BC overhangs used to build level 1 constructs. |
| 202133 | pL0_BC-sec_sig_pep | Level 0 plasmid containing a kozak consensus sequence and a secretion signal peptide sequence with BC overhangs used to build level 1 constructs. |
| 202134 | pL0_BC-mito_sig_pep | Level 0 plasmid containing a kozak consensus sequence and a mitochondria signal peptide sequence with BC overhangs used to build level 1 constructs. |
| 202135 | pL0_BC-nuc_sig_pep | Level 0 plasmid containing a kozak consensus sequence and a nucleus signal peptide sequence with BC overhangs used to build level 1 constructs. |
| 202136 | pL0_BC-ER_sig_pep | Level 0 plasmid containing a kozak consensus sequence and a ER signal peptide sequence with BC overhangs used to build level 1 constructs. |
| 202137 | pL0_CD-GUS | Level 0 plasmid containing a beta glucuronidase reporter gene with CD overhangs used to build a level 1 construct. |
| 202138 | pL0_CD-PT2A | Level 0 plasmid containing a dual 2A (P2A and T2A) peptide sequence with CD overhangs used to build level 1 constructs |
| 202139 | pL0_CD-eYFP | Level 0 plasmid containing an eYFP fluorescent protein with CD overhangs used to build a level 1 construct. |
| 202140 | pL0_CD-roGFP | Level 0 plasmid containing a roGFP fluorescent protein with CD overhangs used to build a level 1 construct. |
| 202141 | pL0_CD-greenlantern | Level 0 plasmid containing a green lantern fluorescent protein with CD overhangs used to build a level 1 construct. |
| 202142 | pL0_CD-eCFP | Level 0 plasmid containing an eCFP fluorescent protein with CD overhangs used to build a level 1 construct. |
| 202143 | pL0_CD-mcherry | Level 0 plasmid containing a mCherry fluorescent protein with CD overhangs used to build a level 1 construct. |
| 202144 | pL0_CD-eYGFP-uv | Level 0 plasmid containing an eYGFP-uv fluorescent protein with CD overhangs used to build a level 1 construct. |
| 202145 | pL0_CD-mRFP1 | Level 0 plasmid containing a mRFP1 fluorescent protein with CD overhangs used to build a level 1 construct. |
| 202146 | pL0_CD-mTurquoise2 | Level 0 plasmid containing a mTurquoise2 fluorescent protein with CD overhangs used to build a level 1 construct. |
| 202147 | pL0_CD-sfGFP | Level 0 plasmid containing a sfGFP fluorescent protein with CD overhangs used to build a level 1 construct. |
| 202148 | pL0_CD-Venus | Level 0 plasmid containing a Venus fluorescent protein with CD overhangs used to build a level 1 construct. |
| 202149 | pL0_CD-eGFP | Level 0 plasmid containing an eGFP fluorescent protein with CD overhangs used to build a level 1 construct. |
| 202150 | pL0_CE-OriT | Level 0 plasmid containing an origin of transfer sequence from pPtPBR11 required for making plasmids mobilizable for conjugation with CE overhangs used to build level 1 constructs. |
| 202151 | pL0_DE-3xStop | Level 0 plasmid containing 3 consecutive stop codons with DE overhangs used to build a level 1 constuct. |
| 202152 | pL0_DE-Per_sig_pep | Level 0 plasmid containing a peroxisome signal peptide sequence with DE overhangs used to build level 1 constructs. |
| 202153 | pL0_DE-mRuby3 | Level 0 plasmid containing a mRuby3 fluorescent protein with DE overhangs used to build a level 1 construct. |
| 202154 | pL0_DE-RFP1 | Level 0 plasmid containing a RFP1 fluorescent protein with DE overhangs used to build a level 1 construct. |
| 202155 | pL0_DE_mTurquoise2 | Level 0 plasmid containing a mTurquoise2 fluorescent protein with DE overhangs used to build a level 1 construct. |
| 202156 | pL0_DE-sfGFP | Level 0 plasmid containing a sfGFP fluorescent protein with DE overhangs used to build a level 1 construct. |
| 202157 | pL0_DE-Venus | Level 0 plasmid containing a Venus fluorescent protein with DE overhangs used to build a level 1 construct. |
| 202158 | pL0_EF-BCA | Level 0 plasmid containing a terminator sequence from gene 51305 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct. |
| 202159 | pL0_EF-47740 | Level 0 plasmid containing a terminator sequence from gene 47740 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct. |
| 202160 | pL0_EF-GEF | Level 0 plasmid containing a terminator sequence from gene 41365 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct. |
| 202161 | pL0_EF-51183 | Level 0 plasmid containing a terminator sequence from gene 51183 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct. |
| 202162 | pL0_EF-27877 | Level 0 plasmid containing a terminator sequence from gene 27877 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct. |
| 202163 | pL0-EF-54730 | Level 0 plasmid containing a terminator sequence from gene 54730 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct. |
| 202164 | pL0_EF-fcpA | Level 0 plasmid containing a terminator sequence from gene 18049 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct. |
| 202165 | pL0_EF-OEE3 | Level 0 plasmid containing a terminator sequence from gene 54499 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct. |
| 202166 | pL0_EF-fbaC5 | Level 0 plasmid containing a terminator sequence from gene 41423 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct. |
| 202167 | pL0_EF-CaMK2 | Level 0 plasmid containing a terminator sequence from gene 39236 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct. |
| 202168 | pL0_EF-sit4 | Level 0 plasmid containing a terminator sequence from gene 48707 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct. |
| 202169 | pL0_EF-NRT | Level 0 plasmid containing a terminator sequence from gene 54101 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct. |
| 202170 | pL0_EF-HO | Level 0 plasmid containing a terminator sequence from gene 5902 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct. |
| 202171 | pL0_EF-act1_tr | Level 0 plasmid containing a terminator sequence from gene 29812 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct. |
| 202172 | pL0_EF-act1_full | Level 0 plasmid containing a terminator sequence from gene 29812 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct. |
| 202173 | pL0_EF-lysA | Level 0 plasmid containing a terminator sequence from gene 21592 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct. |
| 202174 | pL0_EF-vapol | Level 0 plasmid containing a terminator sequence from gene 47434 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct. |
| 202175 | pL0_EF-GPI1 | Level 0 plasmid containing a terminator sequence from gene 23924 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct. |
| 202176 | pL0_EF-P49 | Level 0 plasmid containing a terminator sequence from gene 49202 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct. |
| 202177 | pL0_EF-fcpB | Level 0 plasmid containing a terminator sequence from gene 25172 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct. |
| 202178 | pL0_EF-H4 | Level 0 plasmid containing a terminator sequence from gene 34971 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct. |
| 202179 | pL0_EF-NR | Level 0 plasmid containing a terminator sequence from gene 54983 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct. |
| 202180 | pL0_EF-PtBle | Level 0 plasmid containing a phleomycin antibiotic cassette (fcpF promoter) with EF overhangs used to build a level 1 construct. |
| 202181 | pL0_EF-Fld | Level 0 plasmid containing a terminator sequence from gene 23658 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct. |
| 202182 | pL0_EF-dph1 | Level 0 plasmid containing a terminator sequence from gene 54330 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct. |
| 202183 | pL0_EF-glna | Level 0 plasmid containing a terminator sequence from gene 22357 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct. |
| 202184 | pL0_EF-acc2 | Level 0 plasmid containing a terminator sequence from gene 41316 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct. |
| 202185 | pL0_EF-epyC | Level 0 plasmid containing a terminator sequence from gene 55209 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct. |