Enhancing extracellular vesicle cargo loading and functional delivery by engineering protein-lipid interactions.
Peruzzi JA, Gunnels TF, Edelstein HI, Lu P, Baker D, Leonard JN, Kamat NP
Nat Commun. 2024 Jul 4;15(1):5618. doi: 10.1038/s41467-024-49678-z.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 201536 | pHIE324-ZF6x12-C DsRed-Express2 in TUPV1 | DsRed-Express2 reporter regulated by ZF6 synTF promoter (12x compact binding sites) in TUPV1 for mMoClo golden gate-based assembly |
| 201537 | pPD1178-LPDV promoterless PuroR-P2A-miRFP720 | Landing pad destination vector for mMoClo golden gate-based assembly of landing pad integration vectors; contains BxB1 attB site, promoterless puromycin resistance gene and miRFP720 gene |
| 201539 | pHIE323-LPIV Puro-P2A-miRFP720 ZF6x12-C-DsRed-Express2(x6) phEF1α-BlastR | Landing pad integration vector used to generate ZF6 synTF reporter line (HIE156), assembled with pPD1178 (destination vector), pHIE324-329 (TUPV1-6), pHIE280 (TUPV7), pPD1157 (linker) |
| 202492 | pJPT001-HaloTag-1x FLAG | Express "soluble" HaloTag |
| 202493 | pJPT002-M-HaloTag-1x FLAG | Express "M" HaloTag |
| 202494 | pJPT003-PM-HaloTag-1x FLAG | Express "PM" HaloTag |
| 202495 | pJPT004-1x FLAG-HaloTag-G | Express "G" HaloTag |
| 202496 | pJPT006-1x FLAG-HaloTag-PPF | Express "PPF" HaloTag |
| 202497 | pJPT007-ZF6 synTF-1x FLAG | Express "soluble" synTF (ZF6) |
| 202498 | pJPT008-ZF6 M-synTF-1x FLAG | Express "M" synTF (ZF6) |
| 202499 | pJPT009-ZF6 PM-synTF-1x FLAG | Express "PM" synTF (ZF6) |
| 202500 | pJPT010-ZF6 1x FLAG-synTF-G | Express "G" synTF (ZF6) |
| 202501 | pJPT011-ZF6 1x FLAG-synTF-PPF | Express "PPF" synTF (ZF6) |
| 202502 | pJPT018-LAT_WT-HaloTag-3x FLAG | Express WT-LAT |
| 202503 | pJPT019-LAT_C26A-HaloTag-3x FLAG | Express C26A Lat mutant |
| 202504 | pJPT020-LAT_dCore-HaloTag-3x FLAG | Express dCore Lat mutant |
| 202505 | pJPT021-LAT_HASA-HaloTag-3x FLAG | Express high ASA Lat mutant |
| 202506 | pJPT024-24 Angstrom 4TMD de novo_mRFP1_3x FLAG | Express 24 Angstrom 4TMD de novo protein |
| 202507 | pJPT025-24 Angstrom 12TMD de novo_mRFP1_3x FLAG | Express 24 Angstrom 12TMD de novo protein |
| 202508 | pJPT026-32 Angstrom 4TMD de novo_mRFP1_3x FLAG | Express 32 Angstrom 4TMD de novo protein |
| 202509 | pJPT027-40 Angstrom 4TMD de novo_mRFP1_3x FLAG | Express 40 Angstrom 4TMD de novo protein |
| 202510 | pJPT028-40 Angstrom 12TMD de novo_mRFP1_3x FLAG | Express 40 Angstrom 12TMD de novo protein |