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Affinity proteomics reveals human host factors implicated in discrete stages of LINE-1 retrotransposition.

Taylor MS, LaCava J, Mita P, Molloy KR, Huang CR, Li D, Adney EM, Jiang H, Burns KH, Chait BT, Rout MP, Boeke JD, Dai L
Cell. 2013 Nov 21;155(5):1034-48. doi: 10.1016/j.cell.2013.10.021. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
202589pETM11-6xHis-TEV-hORF1pAllows for IPTG-inducible expression of the human L1RP ORF1 protein in bacteria with an N-terminal 6xHis tag and TEV cleavage site for purification with a minimal scar

Antibodies from Article