Affinity proteomics reveals human host factors implicated in discrete stages of LINE-1 retrotransposition.
Taylor MS, LaCava J, Mita P, Molloy KR, Huang CR, Li D, Adney EM, Jiang H, Burns KH, Chait BT, Rout MP, Boeke JD, Dai L
Cell. 2013 Nov 21;155(5):1034-48. doi: 10.1016/j.cell.2013.10.021.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 202589 | pETM11-6xHis-TEV-hORF1p | Allows for IPTG-inducible expression of the human L1RP ORF1 protein in bacteria with an N-terminal 6xHis tag and TEV cleavage site for purification with a minimal scar |