Substrate binding and coupled mechanisms of Vps4p substrate recruitment and release from autoinhibition.
Wienkers H, Han H, Whitby F, Hill CP
Sci Rep. 2025 Jul 11;15(1):25024. doi: 10.1038/s41598-025-08418-z.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 203356 | 16_GP_Vps4p_L7C_A43C | Vps4 with stabilized MIT domain and 16aa GlyPro N-terminal extension |
| 203357 | Vps4p_L40D | Vps4 with destabilized MIT domain |
| 203358 | 2xPeptide 8_Vps4p_L7C_A43C | Vps4 with stabilized MIT domain with an N-terminal extension of 16 binding amino acids |
| 203360 | Vps4p_Y33D | Vps4 with destabilized MIT domain |
| 203361 | Vps4p_GP_linker | Vps4 with GlyPro linker between MIT domain and Large ATPase Domain |
| 203362 | Vps4p_L7C_A43C | Vps4 with stabilized MIT domain |