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Substrate binding and coupled mechanisms of Vps4p substrate recruitment and release from autoinhibition.

Wienkers H, Han H, Whitby F, Hill CP
Sci Rep. 2025 Jul 11;15(1):25024. doi: 10.1038/s41598-025-08418-z. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
20335616_GP_Vps4p_L7C_A43CVps4 with stabilized MIT domain and 16aa GlyPro N-terminal extension
203357Vps4p_L40DVps4 with destabilized MIT domain
2033582xPeptide 8_Vps4p_L7C_A43CVps4 with stabilized MIT domain with an N-terminal extension of 16 binding amino acids
203360Vps4p_Y33DVps4 with destabilized MIT domain
203361Vps4p_GP_linkerVps4 with GlyPro linker between MIT domain and Large ATPase Domain
203362Vps4p_L7C_A43CVps4 with stabilized MIT domain

Antibodies from Article