Multiplexed volumetric CLEM enabled by antibody derivatives provides new insights into the cytology of the mouse cerebellar cortex.
Han X, Lu X, Li PH, Wang S, Schalek R, Meirovitch Y, Lin Z, Adhinarta J, Berger D, Wu Y, Fang T, Meral ES, Asraf S, Ploegh H, Pfister H, Wei D, Jain V, Trimmer JS, Lichtman JW
bioRxiv. 2023 Jun 8:2023.05.20.540091. doi: 10.1101/2023.05.20.540091. Preprint. PubMed Article
Han X, Lu X, Li PH, Wang S, Schalek R, Meirovitch Y, Lin Z, Adhinarta J, Berger D, Wu Y, Fang T, Meral ES, Asraf S, Ploegh H, Pfister H, Wei D, Jain V, Trimmer JS, Lichtman JW
bioRxiv. 2023 Jun 8:2023.05.20.540091. doi: 10.1101/2023.05.20.540091. Preprint. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 204419 | anti-GFP scFv [N86/38] with sortase tag | Mammalian Expression of anti-GFP scFv with a sortase tag for direct dye conjugation. Derived from hybridoma N86/38. |
| 204420 | anti-GFAP R416WT scFv [N206B/9] with sortase tag | Mammalian Expression of anti-GFAP scFv with a sortase tag for direct dye conjugation. Derived from hybridoma N206B/9. |
| 204421 | anti-Calbindin scFv [L109/57] with sortase tag | Mammalian Expression of anti-Calbindin scFv with a sortase tag for direct dye conjugation. Derived from hybridoma L109/57. |
| 204422 | anti-PSD-95 scFv [K28/43] with sortase tag | Mammalian Expression of anti-PSD-95 scFv with a sortase tag for direct dye conjugation. Derived from hybridoma K28/43. |
| 204423 | anti-Parvalbumin scFv [L114/81] with sortase tag | Mammalian Expression of anti-Parvalbumin scFv with a sortase tag for direct dye conjugation. Derived from hybridoma L114/81. |
| 204424 | anti-VGluT1 scFv [N28/9] with sortase tag | Mammalian Expression of anti-VGluT1 scFv with a sortase tag for direct dye conjugation. Derived from hybridoma N28/9. |
| 204425 | anti-Kv1.2 scFv [K14/16] with sortase tag | Mammalian Expression of anti-Kv1.2 scFv with a sortase tag for direct dye conjugation. Derived from hybridoma K14/16. |
| 204426 | anti-NPY/Neuropeptide Y scFv [L115/13] with sortase tag | Mammalian Expression of anti-Neuropeptide Y scFv with a sortase tag for direct dye conjugation. Derived from hybridoma L115/13. |