Discovery of L-threonine transaldolases for enhanced biosynthesis of beta-hydroxylated amino acids.

Jones MA, Butler ND, Anderson SR, Wirt SA, Govil I, Lyu X, Fang Y, Kunjapur AM
Commun Biol. 2023 Sep 11;6(1):929. doi: 10.1038/s42003-023-05293-0. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
204629	P14-pZE-SUMO-ObiH	ColE1 ori, KanR, TetR, Tet promoter with a codon optimized obiH gene bearing an N-terminal hexahistidine tag followed by a SUMO tag and a TEV protease cleavage site.
204630	P15-pZE-SUMO-PiTTA	ColE1 ori, KanR, TetR, Tet promoter with a codon optimized piTTA gene bearing an N-terminal hexahistidine tag followed by a SUMO tag and a TEV protease cleavage site.
204631	P17-pZE-SUMO-CsTTA	ColE1 ori, KanR, TetR, Tet promoter with a codon optimized csTTA gene bearing an N-terminal hexahistidine tag followed by a SUMO tag and a TEV protease cleavage site.
204632	P18-pZE-SUMO-BuTTA	ColE1 ori, KanR, TetR, Tet promoter with a codon optimized buTTA gene bearing an N-terminal hexahistidine tag followed by a SUMO tag and a TEV protease cleavage site.
204633	P24-pZE-SUMO-KaTTA	ColE1 ori, KanR, TetR, Tet promoter with a codon optimized kaTTA gene bearing an N-terminal hexahistidine tag followed by a SUMO tag and a TEV protease cleavage site.
204634	P25-pZE-SUMO-PbTTA	ColE1 ori, KanR, TetR, Tet promoter with a codon optimized pbTTA gene bearing an N-terminal hexahistidine tag followed by a SUMO tag and a TEV protease cleavage site.