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Single-molecule magnetic tweezers to probe the equilibrium dynamics of individual proteins at physiologically relevant forces and timescales.

Tapia-Rojo R, Mora M, Garcia-Manyes S
Nat Protoc. 2024 Mar 11. doi: 10.1038/s41596-024-00965-5. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
206039pFN18A-HaloTag-BiotinHaloTag-(Ig32)2-Talin R3-IVVI-(Ig32)2-10xHisTag-AviTag
206041pFN18A-HaloTag-SpyCatcherHaloTag-Spy0128-(Protein of Interest)-Spy0128-SpyCatcher-8xHisTag

Antibodies from Article