A phosphorylation-controlled switch confers cell cycle-dependent protein relocalization.
Cao X, Huang S, Wagner MM, Cho YT, Chiu DC, Wartchow KM, Lazarian A, McIntire LB, Smolka MB, Baskin JM
Nat Cell Biol. 2024 Oct;26(10):1804-1816. doi: 10.1038/s41556-024-01495-8. Epub 2024 Aug 29.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 205232 | MARS | Expresses PLEKHA5 aa 143-271 (K163A and R164A) fused to mScarlet-i in mammalian cells |
| 205233 | 2xMARS | Expresses two repeats of PLEKHA5 aa 143-271 (K163A and R164A) fused to mScarlet-iin mammalian cells |
| 205234 | MARS-nGFP | Expresses PLEKHA5 aa 143-271 (K163A and R164A) fused to a HA-tagged GFP nanobody in mammalian cells |
| 205235 | 2xMARS-nGFP | Expresses two repeats of PLEKHA5 aa 143-271 (K163A and R164A) fused to a HA-tagged GFP nanobody in mammalian cells |
| 205236 | pCDNA3-MARS-p85(iSH2) | Expresses PLEKAH5 aa 143-271 (K163A and R164A) fused to the iSH2 domain of mouse p85beta |
| 205237 | pCDNA3-2xMARS-PLD | Expresses two repeats of PLEKAH5 aa 143-271 (K163A and R164A) fused to phospholipase D from Streptomyces sp. PMF |
| 205238 | pCDH-2xMARS-nGFP-puro | Lentiviral vector encoding two repeats of PLEKHA5 aa 143-271 (K163A and R164A) fused to a HA-tagged GFP nanobody |
| 205239 | pSBtet-2xMARS-nGFP-puro | DOX inducible expression of two repeats of PLEKHA5 aa 143-271 (K163A and R164A) fused to a HA-tagged GFP nanobody in mammalian cells from the Sleeping Beauty transposon system |