Fanzor is a eukaryotic programmable RNA-guided endonuclease.
Saito M, Xu P, Faure G, Maguire S, Kannan S, Altae-Tran H, Vo S, Desimone A, Macrae RK, Zhang F
Nature. 2023 Jun 28. doi: 10.1038/s41586-023-06356-2.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 205252 | pMJ45: pRS426-His-MBP-SpuFz1_Native | S. cerevisiae expression vector for SpuFz1 native guide |
| 205253 | pMJ106: pRS426-His-MBP-NlovFz2_Native | S. cerevisiae expression vector for NlovFz2 native guide |
| 205254 | pMJ235: pRS426-His-MBP-GtFz1_Native | S. cerevisiae expression vector for GtFz1 native guide |
| 205255 | pMJ240: pRS426-His-MBP-MmeFz2_Native | S. cerevisiae expression vector for MmeFz2 native guide |
| 205256 | pMJ123: pRS426-His-MBP-SpuFz1_PSP1 | S. cerevisiae expression vector for SpuFz1 reprogrammed guide (targeting PSP1) |
| 205257 | pMJ127: pRS426-His-MBP-NlovFz2_PSP1 | S. cerevisiae expression vector for NlovFz2 reprogrammed guide (targeting PSP1) |
| 205258 | pMJ273: pRS426-His-MBP-GtFz1_PSP1 | S. cerevisiae expression vector for GtFz1 reprogrammed guide (targeting PSP1) |
| 205260 | pMSSM24: pCMV-SpuFz1 | Human expression vector for human codon optimized SpuFz1 |
| 205261 | pMJ162: pU6-SpuFz1_omegaRNA(75nt)-entry | Human expression vector for SpuFz1 omegaRNA 75-nt scaffold (BbsI guide entry site) |
| 205263 | pMSSM151: pU6-NlovFz2_omegaRNA-entry | Human expression vector for NlovFz2 omegaRNA (BsmBI guide entry site) |
| 205264 | pMJ326: pCMV-GtFz1 | Human expression vector for human codon optimized GtFz1 |
| 205265 | pMJ332: pU6-GtFz1_omegaRNA-entry | Human expression vector for GtFz1 omegaRNA (BbsI guide entry site) |
| 205266 | pMJ329: pCMV-MmeFz2 | Human expression vector for human codon optimized MmeFz2 |
| 205267 | pMJ333: pU6-MmeFz2_omegaRNA-entry | Human expression vector for MmeFz2 omegaRNA (BbsI guide entry site) |
| 205268 | pMJ204: pU6-SpuFz1_omegaRNA(350nt)-entry | Human expression vector for SpuFz1 omegaRNA 5' +275 nt extended scaffold (BbsI guide entry site) |
| 205269 | pMJ548: pU6-SpuFz1_MS2-omegaRNA(75nt)-entry | Human expression vector for SpuFz1 omegaRNA 75-nt scaffold + 5' MS2- (BbsI guide entry site) |
| 205271 | pMSSM16: pCMV-HA-NLS-SpuFz1 | Human expression vector for human codon optimized N-terminal NLS-tagged SpuFz1 |
| 205272 | pMSSM59: pCMV-HA-NLS-SpuFz1-D300R | Human expression vector for human codon optimized N-terminal NLS-tagged SpuFz1-D300R |
| 205274 | pMSSM58: pCMV-HA-NLS-SpuFz1-D487K | Human expression vector for human codon optimized N-terminal NLS-tagged SpuFz1-D487K |
| 205275 | pMSSM97: pCMV-HA-NLS-SpuFz1-E498R | Human expression vector for human codon optimized N-terminal NLS-tagged SpuFz1-E498R |
| 205276 | pMSSM105: pCMV-HA-NLS-SpuFz1-T513K | Human expression vector for human codon optimized N-terminal NLS-tagged SpuFz1-T513K |
| 205277 | pMSSM110: pCMV-HA-NLS-SpuFz1-v2 | Human expression vector for human codon optimized N-terminal NLS-tagged SpuFz1-v2 |
| 205278 | pMJ529: pU6-SpuFz1_MS2-omegaRNA(75nt)-Guide(B2M) | Human expression vector for SpuFz1 omegaRNA 75-nt scaffold + 5' MS2- (Guide:B2M) |