Staphylococcus aureus sensor histidine kinase VraS mutations linked to antibiotic resistance affect enzymatic catalysis, stability, and substrate interactions.
Cagle-White B, Bhattarai S, Sunderhaus A, Scott E, Solis A, Marsh L, Catague KL, Sarkar A, Abdel Aziz MH
Arch Biochem Biophys. 2026 Feb;776:110715. doi: 10.1016/j.abb.2025.110715. Epub 2025 Dec 24.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 206333 | pET15-VraS - A314T | Bacterial Expression of A314T VraS |
| 206334 | pET15-VraS - D242G | Bacterial Expression of D242G VraS |
| 206335 | pET15-VraS - E276K | Bacterial Expression of E276K VraS |
| 206336 | pET15-VraS - L114S | Bacterial Expression of L114S VraS |
| 206337 | pET15-VraS - M192I | Bacterial Expression of M192I VraS |
| 206338 | pET15-VraS - S329L | Bacterial Expression of S329L VraS |
| 206873 | pET15-VraS - L114S/D242G | Bacterial Expression of VraS double mutant L114S/D242G |