A versatile CRISPR-based system for lineage tracing in living plants.

Dona M, Bradamante G, Bogojevic Z, Gutzat R, Streubel S, Mosiolek M, Dolan L, Mittelsten Scheid O
Plant J. 2023 Jul 5. doi: 10.1111/tpj.16378. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
210528	pGGA-pCLV3	Cloning A. thaliana CLV3 promoter as GreenGate module A
210533	pGGB-SpCas9	Cloning A.thaliana codon optimized SpCas9 CDS as GreenGate module B
210536	pGGC-PEA3AT	Cloning P. sativum PEA3A terminator as GreenGate module C
210539	pGGC-LexACasPea	Cloning estradiol-inducible expression cassette containi LexA XVE binding sequence followed by SpCas9 CDS and PEA3AT as GreenGate module C
210542	pGGE1-HTR5p	Cloning A. thaliana HTR5 promoter as GreenGate module E1
210548	pCLV3Cas-LT0	Binary vector expressing the Lineage Tracing system. Cas9 expression driven by CLV3 promoter. LT0 reporter
210549	pCLV3Cas-LT1	Binary vector expressing the Lineage Tracing system. Cas9 expression driven by CLV3 promoter. LT1 reporter
210550	pCLV3Cas-LT2	Binary vector expressing the Lineage Tracing system. Cas9 expression driven by CLV3 promoter. LT1 reporter
210551	LT0	Binary vector expressing the Lineage Tracing system. No Cas9 control expression cassette. LT0 reporter
210552	LT1	Binary vector expressing the Lineage Tracing system. No Cas9 control expression cassette. LT1 reporter
210553	LT2	Binary vector expressing the Lineage Tracing system. No Cas9 control expression cassette. LT2 reporter
210555	ipGL2Cas	Binary vector expressing the estradiol-inducible Lineage Tracing system. XVE expression driven by GL2 promoter.
210556	pEF1Cas-LT1	Binary vector expressing the Lineage Tracing system. Cas9 expression driven by M. polymorpha EF1 promoter. LT1 reporter under control of MpEF1p
210559	ipYUC2Cas-LT1	Binary vector expressing the estradiol-inducible Lineage Tracing system. XVE expression driven by M. polymorpha YUC2 promoter.