CaBLAM: a high-contrast bioluminescent Ca(2+) indicator derived from an engineered Oplophorus gracilirostris luciferase.
Lambert GG, Crespo EL, Murphy J, Turner KL, Gershowitz E, Cunningham M, Boassa D, Luong S, Celinskis D, Allen JJ, Venn S, Zhu Y, Karadas M, Chen J, Marisca R, Gelnaw H, Nguyen DK, Hu J, Sprecher BN, Tree MO, Orcutt R, Heydari D, Bell AB, Torreblanca-Zanca A, Hakimi A, Czopka T, Shoham S, Nagel KI, Schoppik D, Andrade A, Lipscombe D, Moore CI, Hochgeschwender U, Shaner NC
Nat Methods. 2025 Dec 2. doi: 10.1038/s41592-025-02972-0.
(Link opens in a new window)
PubMed
(Link opens in a new window)
Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 244128 | pCAG-GeNL_SS | Sensor scaffold luciferase derived from Oplophorus luc. |
| 244129 | pCAG-CaBLAM | Bioluminescent calcium indicator |
| 244130 | pCAG-CaBLAM_294W | Lower affinity variant of CaBLAM bioluminescent calcium indicator |
| 244131 | pCAG-CaBLAM_332W | Higher affinity variant of CaBLAM bioluminescent calcium indicator |
| 244227 | pAAV hSyn CaBLAM | Bioluminescent reporter for calcium signaling in neurons |
| 244228 | pAAV GFAP CaBLAM | Bioluminescent reporter for calcium signaling in astrocytes |
| 244229 | pAAV EfIa DIO CaBLAM | Cre dependent bioluminescent reporter for calcium signaling |