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RUNX1 isoform disequilibrium promotes the development of trisomy 21-associated myeloid leukemia.

Gialesaki S, Brauer-Hartmann D, Issa H, Bhayadia R, Alejo-Valle O, Verboon L, Schmell AL, Laszig S, Regenyi E, Schuschel K, Labuhn M, Ng M, Winkler R, Ihling C, Sinz A, Glass M, Huttelmaier S, Matzk S, Schmid L, Struwe FJ, Kadel SK, Reinhardt D, Yaspo ML, Heckl D, Klusmann JH
Blood. 2023 Mar 9;141(10):1105-1118. doi: 10.1182/blood.2022017619. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
181976pLeGO.sgGata1.4.RUNX1A.iG2gene knock out of mouse GATA1, overexpression of 3xFLAG-RUNX1A (human)
181977SIN40C.TRE.HA.RUNX1A.IRES.dTomato.PGK.sfGFP.P2A.Tet3GHuman RUNX1A gene overexpression
181978SIN40C.TRE.HA.RUNX1C.IRES.dTomato.PGK.sfGFP.P2A.Tet3GHuman RUNX1C gene overexpression
181979pRRL.PPT.SFFV.HA.RUNX1A.IRES.eGFPHuman RUNX1A gene overexpression
181980pRRL.PPT.SFFV.HA.RUNX1C.IRES.eGFPHuman RUNX1C gene overexpression
181981pLKO5d.SFFV.Cas9.P2A.BSDLentiviral Cas9 expression
187191pLKO5d.SFFV.IRES.dTomato Lentiviral gene overexpression (backbone/control)
187192pLKO5d.SFFV.Cre.IRES.dTomato Lentiviral overexpression of Cre recombinase

Antibodies from Article