Fluorescent reporters give new insights into antibiotics-induced nonsense and frameshift mistranslation.
Hinnu M, Putrins M, Kogermann K, Kaldalu N, Tenson T
Sci Rep. 2024 Mar 22;14(1):6883. doi: 10.1038/s41598-024-57597-8.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 208183 | pSC101-GFPmut2-mScarlet-I | mistranslation GFP and mScarlet-I positive control |
| 208184 | pSC101-AmpR EV | empty plasmid vector, non-fluorescent |
| 208185 | pSC101-GFPmut2(ΔmScarlet) | GFP positive, mScarlet-I negative control |
| 208186 | pSC101-GFPmut2-mScarlet-I 6Trp7Ser | mistranslation GFP and mScarlet-I positive control with Trp insertion |
| 208187 | pSC101-GFPmut2-mScarlet-I TAA | nonsense mistranslation, TAA stop |
| 208188 | pSC101-GFPmut2-mScarlet-I TAG | nonsense mistranslation, TAG stop |
| 208189 | pSC101-GFPmut2-mScarlet-I TGA | nonsense mistranslation, TGA stop |
| 208190 | pSC101-GFPmut2-mScarlet-I TGATC | nonsense mistranslation, TGA stop variant 2 |
| 208191 | pSC101-GFPmut2-mScarlet-I T7 | frameshift mistranslation, T7 |
| 208192 | pSC101-GFPmut2-mScarlet-I A5 | frameshift mistranslation, A5 |
| 208193 | pSC101-GFPmut2-mScarlet-I A4 | frameshift mistranslation, A4 |
| 208194 | pSC101-GFPmut2-mScarlet-I A6T | frameshift mistranslation, A6T |
| 208195 | pSC101-GFPmut2-mScarlet-I C3T | frameshift mistranslation, C3T |