Molecular consequences of acute versus chronic CDK12 loss in prostate carcinoma nominates distinct therapeutic strategies
Frank S, Persse T, Coleman I, Bankhead III A, Li D, De-Sarkar N, Wilson D, Rudoy D, Vashisth M, Galipeau P, Yang M, Hanratty B, Dumpit R, Morrissey C, Corey E, Montgomery RB, Haffner MC, Pritchard CC, Vasioukhin V, Ha G, Nelson PS
eLife 2025
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 208342 | pLenti-Cas9.mCherry | Lentivirus with EF1a driven Cas9 linked (P2A) to mCherry. |
| 208343 | pLCV2-AAVS1(hU6-sg1-mU6-sg2)-Blast | pLentiCRISPRv2-Blast backbone with two separate sgRNAs against AAVS1. Serves as a negative control by targeting a safe harbor locus. |
| 208344 | pLCV2-CDK12(hU6-sg2-mU6-sg3)-Blast | pLentiCRISPRv2-Blast backbone with two separate sgRNAs against CDK12 |
| 208346 | pCR8-CDK12(sgR) | Gateway cloning entry vector with CDK12 with sgRNA resistant silent mutations. Must be recombined into a DEST vector for expression. |
| 208347 | pCR8-CDK12 | Gateway cloning entry vector with CDK12. Must be recombined into a DEST vector for expression. |
| 208348 | pLCV2-CDK13(hU6-sg2-mU6-sg3)-Blast | pLentiCRISPRv2-Blast backbone with two separate sgRNAs against CDK13 |
| 208349 | pLenti-sgStuffer-GFP-Puro | Lentivirus sgRNA backbone with GFP and PuroR. Compatible with golden gate cloning (Esp3i/BsmBI) |
| 208350 | pLenti-sgStuffer-mCherry-Puro | Lentivirus sgRNA backbone with mCherry and PuroR. Compatible with golden gate cloning (Esp3i/BsmBI) |
| 208408 | FUCGW-DEST | Lentivirus compatible with LR Gateway cloning for UBC-driven gene expression with CMV-GFP in backbone |
| 208409 | FUCRW-DEST | Lentivirus compatible with LR Gateway cloning for UBC-driven gene expression with CMV-RFP in backbone |