Single-step genome engineering in the bee gut symbiont Snodgrassella alvi
Lariviere PJ, Zuberi Ashraf AHM, Leonard SP, Miller LG, Moran NA, Barrick JE
bioRxiv 2023.09.19.558440
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 209540 | pBTK1050 | BTK Type 1 homology arm for Golden Gate assembly |
| 209541 | pBTK1051 | BTK Type 5 homology arm for Golden Gate assembly |
| 209542 | pBTK1052 | BTK Type 2 antibiotic resistance cassette for Golden Gate assembly |
| 209543 | pBTK1053 | BTK Type 3-4 fluorescent protein cassette for Golden Gate assembly |
| 209544 | pBTK1054 | BTK assembled plasmid for integrating a KanR+E2-Crimson cassette into the S. alvi genome at a neutral site |
| 209546 | pBTK1056 | BTK Type 4 antibiotic resistance cassette for Golden Gate assembly |
| 209547 | pBTK1057 | BTK assembled plasmid for integrating a KanR+GFP cassette into the S. alvi genome at a neutral site |
| 209548 | pBTK1058 | BTK Type 1 homology arm for Golden Gate assembly |
| 209549 | pBTK1059 | BTK Type 5 homology arm for Golden Gate assembly |
| 209550 | pBTK1061 | BTK assembled plasmid for integrating a KanR+E2-Crimson cassette into the S. alvi genome in place of the clpA gene |