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Remote sensing of endogenous pigmentation by inducible synthetic circuits in grasses

Lee DY, Acosta-Gamboa L, Saleh L, Pathak S, Swyers N, Morgan A, Meerdink S, Kuzio C, Calderon S, Sheng H, Kenney S, Zare A, Gehan M, Nusinow DA
bioRxiv 2025.06.20.660755 (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
210753pDL0_012PU_ Oryza sativa alpha-tubulin A1 terminator
210754pDL0_011PU_Zea mays Ubiquitin1 promoter
210755pDL0_033PU_Zea mays EF1a promoter
210756pDL0_032PU_Oryza sativa Ubiquitin3 promoter
210759pDL0_028PU_6 x Lac operator sequence 35S minimal fusion promoter (pOp6)
210760pDL0_003SC_Hygromycin phosphotransferase
210761pDL0_029SC_Oryza sativa codon-optimized LHGR-N(Ligand binding domain and rat glucocorticoid receptor (rcoLHGR-N)
210762pDL0_138SC_Setaria viridis R1 coding sequence
210763pDL0_013T_Oryza sativa alpha-tubulin A1 promoter_R
210764pDL0_044T_Arabidopsis thaliana heat shock protein 18.2 terminator (HspT)
210765pDL0_009T_Octopine synthase terminator
210766pDL0_042T_CaMV 35S polyA signal sequence
210769pDL0_005T_Nopaline synthase terminator
210770pDL0_159S_Setaria viridis R1 coding sequence
210771pDL0_176S_Start codon
210772pDL0_217S_Setaria viridis C1 coding sequence
210773pDL0_267C_Stop codon
210774pDL0_163C1_Porcine techovirus-1 P2A skipping peptide (P2A)
210775pDL0_162C1_Equine rhinitis A virus_E2A skipping peptide (E2A)
210776pDL0_180C1_Setaria viridis C1 coding sequence
210777pDL0_119C2_6xHis_TEV_3xFlag C' terminus Tag (HFC)
210778pDL0_212C2_Pyrearinus termitilluminans click beetle luciferase ('Eluc' version - human-codon optimized)
210779pDL0_158PUSCT_Drosophila melanogaster Gypsy-insulator sequence
210780pDL0_243C2_Endliker for the extension of cloning using pLac:AmilGFP as a visible marker for the cloning
210864pDL0_117SC_Photinus pryalis Firefly Luciferase coding sequence
210865pDL0_198C1_Pyrearinus termitilluminans beetle luciferase ('Eluc' version - human-codon optimized)
211335pDL0_175C2_Stop linker

Antibodies from Article