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Polyglycine-mediated aggregation of FAM98B disrupts tRNA processing in GGC repeat disorders.

Yang J, Xu Y, Ziehr DR, Taylor MS, Valenstein ML, Frenkel EM, Bush JR, Rutter K, Stevanovski I, Shi CY, Kesavan M, Pinto RM, Deveson I, Bartel DP, Sabatini DM, Chivukula RR
Science. 2025 Jul 17;389(6757):eado2403. doi: 10.1126/science.ado2403. Epub 2025 Jul 17. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
211345pDOXOFF 15xGlyDOX-regulated lentiviral expression of 15xGly-EGFP-3xHA
211350pLJC5 mScarlet-FAM98BConstitutive lentiviral expression of V5-mScarlet-FAM98B fusion protein
211353pLJY1 DD-FAM98B[1-330]Constitutive lentiviral expression of DD-V5-FAM98B[1-330] fusion protein
211354pRK5R MCS+empty vector control
211355pRK5R 15xGlyTransient expression of 15xGly-EGFP-3xHA
211357pRK5R DD-FAM98BTransient expression of DD-V5-FAM98B
211358pRK5R DD-FAM98B[1-330]Transient expression of DD-V5-FAM98B[1-330]
211359pTRNA scPhe-GAATransient expression of scPhe-GAA
242191pRK5R 15xGly-uFMR1-3xHATransient expression of 15xGly-uFMR1-3xHA

Antibodies from Article