Polyglycine-mediated aggregation of FAM98B disrupts tRNA processing in GGC repeat disorders
Yang J, Xu Y, Ziehr DR, Taylor MS, Valenstein ML, Frenkel EM, Bush JR, Rutter K, Stevanovski I, Shi CY, Kesavan M, Pinto RM, Deveson I, Bartel DP, Sabatini DM, Chivukula RR
Science 17 July 2025, 389(6757). doi: 10.1126/science.ado2403
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 211345 | pDOXOFF 15xGly | DOX-regulated lentiviral expression of 15xGly-EGFP-3xHA |
| 211353 | pLJY1 DD-FAM98B[1-330] | Constitutive lentiviral expression of DD-V5-FAM98B[1-330] fusion protein |
| 211354 | pRK5R MCS+ | empty vector control |
| 211355 | pRK5R 15xGly | Transient expression of 15xGly-EGFP-3xHA |
| 211358 | pRK5R DD-FAM98B[1-330] | Transient expression of DD-V5-FAM98B[1-330] |
| 211359 | pTRNA scPhe-GAA | Transient expression of scPhe-GAA |