Polyglycine-mediated aggregation of FAM98B disrupts tRNA processing in GGC repeat disorders.
Yang J, Xu Y, Ziehr DR, Taylor MS, Valenstein ML, Frenkel EM, Bush JR, Rutter K, Stevanovski I, Shi CY, Kesavan M, Pinto RM, Deveson I, Bartel DP, Sabatini DM, Chivukula RR
Science. 2025 Jul 17;389(6757):eado2403. doi: 10.1126/science.ado2403. Epub 2025 Jul 17.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 211345 | pDOXOFF 15xGly | DOX-regulated lentiviral expression of 15xGly-EGFP-3xHA |
| 211350 | pLJC5 mScarlet-FAM98B | Constitutive lentiviral expression of V5-mScarlet-FAM98B fusion protein |
| 211351 | pLJC5 mScarlet-FAM98B[1-330] | Constitutive lentiviral expression of V5-mScarlet-FAM98B[1-330] fusion protein |
| 211353 | pLJY1 DD-FAM98B[1-330] | Constitutive lentiviral expression of DD-V5-FAM98B[1-330] fusion protein |
| 211354 | pRK5R MCS+ | empty vector control |
| 211355 | pRK5R 15xGly | Transient expression of 15xGly-EGFP-3xHA |
| 211357 | pRK5R DD-FAM98B | Transient expression of DD-V5-FAM98B |
| 211358 | pRK5R DD-FAM98B[1-330] | Transient expression of DD-V5-FAM98B[1-330] |
| 211359 | pTRNA scPhe-GAA | Transient expression of scPhe-GAA |
| 242191 | pRK5R 15xGly-uFMR1-3xHA | Transient expression of 15xGly-uFMR1-3xHA |