Recombinant biosensors for multiplex and super-resolution imaging of phosphoinositides.
Maib H, Adarska P, Hunton R, Vines JH, Strutt D, Bottanelli F, Murray DH
J Cell Biol. 2024 Jun 3;223(6):e202310095. doi: 10.1083/jcb.202310095. Epub 2024 Apr 5.
(Link opens in a new window)
PubMed
(Link opens in a new window)
Article
A subset of these plasmids is included in the Phosphoinositide Biosensors Kit.
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 211507 | 6xHis-SNAP-PI Probe | For expression of recombinant biosensor for detection of Phosphatidylinositol (PI) from bacteria |
| 211508 | 6xHis-SNAP-PI(3)P Probe | For expression of recombinant biosensor for detection of Phosphatidylinositol-3-Phosphate (PI(3)P) from bacteria |
| 211509 | 6xHis-SNAP-PI(4)P Probe | For expression of recombinant biosensor for detection of Phosphatidylinositol-4-Phosphate (PI(4)P) from bacteria |
| 211511 | 6xHis-SNAP-PI(3,4)P2 Probe | For expression of recombinant biosensor for detection of Phosphatidylinositol-3,4-Bisphosphate (PI(3,4)P2) from bacteria |
| 211512 | 6xHis-SNAP-PI(3,5)P2 Probe | For expression of recombinant biosensor for detection of Phosphatidylinositol-3,5-Bisphosphate (PI(3,5)P2) from bacteria |
| 211513 | 6xHis-SNAP-PI(4,5)P2 Probe | For expression of recombinant biosensor for detection of Phosphatidylinositol-4,5-Bisphosphate (PI(4,5)P2) from bacteria |
| 211514 | 6xHis-SNAP-PI(3,4,5)P3 Probe | For expression of recombinant biosensor for detection of Phosphatidylinositol-3,4,5-Trisphosphate (PI(3,4,5)P3) from bacteria |