NIS-Seq enables cell-type-agnostic optical perturbation screening.
Fandrey CI, Jentzsch M, Konopka P, Hoch A, Blumenstock K, Zackria A, Maasewerd S, Lovotti M, Lapp DJ, Gohr FN, Suwara P, Swiezewski J, Rossnagel L, Gobs F, Cristodaro M, Muhandes L, Behrendt R, Lam MC, Walgenbach KJ, Bald T, Schmidt FI, Latz E, Schmid-Burgk JL
Nat Biotechnol. 2024 Dec 19. doi: 10.1038/s41587-024-02516-5.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 211699 | CROPseq-iT7 | NIS-Seq-compatible lentiviral vector for expressing sgRNAs |
| 223064 | CROPseq-iT7 Human CRISPR Knockout Pooled Library (Brunello) | Optical pooled genome-scale CRISPR KO screening using Nuclear In-Situ Sequencing (NIS-Seq) |