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SWITCHER, a CRISPR-inducible floxed wild-type Cre regulating CRISPR activity.

Schuster B, Dobiasovska I, Curcic J, Pajer P, Borna S, Bartunek P
Commun Biol. 2025 Jul 2;8(1):982. doi: 10.1038/s42003-025-08427-8. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
213043pSWITCHER-TF1-dsREDSWITCHER ready LoxP-EGFP reporter plasmid for cloning of a customized CRISPR-inducible Cre-construct
213044pSplit-SWITCHER1-TF1-dsREDA CRISPR/Cas12a-inducible Split-Cre construct with dsRED as a reporter after the Lox-Stop-Lox cassette
213045pSplit-SWITCHER2-TF1-dsREDA CRISPR/Cas12a-inducible Split-Cre construct with dsRED as a reporter after the Lox-Stop-Lox cassette
213046pU6-LbCpf1-crF1Guide RNA expression vector with the synthetic crRNA crF1 targeting the SWITCHER construct with the target site TF1
213047pU6-LbCpf1-crR2Guide RNA expression vector with the synthetic crRNA crR2 targeting the SWITCHER construct with the target site TR2
213048pLoxP-EGFP-crRNA-entrySWITCHER ready LoxP-EGFP reporter plasmid for cloning of a customized CRISPR-inducible Cre-construct

Antibodies from Article