SWITCHER, a CRISPR-inducible floxed wild-type Cre regulating CRISPR activity.
Schuster B, Dobiasovska I, Curcic J, Pajer P, Borna S, Bartunek P
Commun Biol. 2025 Jul 2;8(1):982. doi: 10.1038/s42003-025-08427-8.
(Link opens in a new window)
PubMed
(Link opens in a new window)
Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 213043 | pSWITCHER-TF1-dsRED | SWITCHER ready LoxP-EGFP reporter plasmid for cloning of a customized CRISPR-inducible Cre-construct |
| 213044 | pSplit-SWITCHER1-TF1-dsRED | A CRISPR/Cas12a-inducible Split-Cre construct with dsRED as a reporter after the Lox-Stop-Lox cassette |
| 213045 | pSplit-SWITCHER2-TF1-dsRED | A CRISPR/Cas12a-inducible Split-Cre construct with dsRED as a reporter after the Lox-Stop-Lox cassette |
| 213046 | pU6-LbCpf1-crF1 | Guide RNA expression vector with the synthetic crRNA crF1 targeting the SWITCHER construct with the target site TF1 |
| 213047 | pU6-LbCpf1-crR2 | Guide RNA expression vector with the synthetic crRNA crR2 targeting the SWITCHER construct with the target site TR2 |
| 213048 | pLoxP-EGFP-crRNA-entry | SWITCHER ready LoxP-EGFP reporter plasmid for cloning of a customized CRISPR-inducible Cre-construct |