Kang Zhou Lab: Harnessing xylitol utilization in Escherichia coli for antibiotic-free plasmid and recombinant protein production.

Unpublished

Plasmids from Article

ID	Plasmid	Purpose
212138	INT_Cargo_PgyrA_gfp	Cargo plasmid for integrating PgyrA expression cassette of green fluorescent protein in genome.Plasmid can be removed by incubating cells at 37 C.
212139	INT_Cargo_T7_gutB	Cargo plasmid for integrating PT7 expression cassette of xylitol dehydrogenase in in genome.Plasmid can be removed by incubating cells at 37 C.
212142	INT_Cargo_PgyrA_katG_dadA6	Cargo plasmid for integrating PgyrA expression cassette of catalase-peroxidase and D-amino acid dehydrogenase into the genome. Plasmid can be removed by incubating cells at 37 C.
212143	INT_GFPsp_CamRcargo	Preparatory plasmid for gene disruption into the genome using CRISPR-Cas12a. Plasmid can be removed by incubating cells at 37 C.
212144	INT_GFPsp	Preparatory plasmid for operon integration into the genome using CRISPR-Cas12a. Plasmid can be removed by incubating cells at 37 C.
212145	INT_SS9sp	Effector plasmid for for operon integration into the genome (Safe Site 9) using CRISPR-Cas12a. Plasmid can be removed by incubating cells at 37 C.
212146	PgyrA_gfp	Plasmid expressing green fluorescent protein under constitutive promoter PgyrA control.
212148	pGU06	Plasmid expressing alanine-glyoxylate aminotransferase, glutamate-pyruvate aminotransferase and tyrosine aminotransferase under constitutive promoter PgyrA control.
212149	pGU08	Plasmid expressing catalase-peroxidase and D-amino acid dehydrogenase under constitutive promoter PgyrA control. Best E. coli glycine utilization plasmid.
212150	pCT03	Plasmid expressing alanine dehydrogenase under inducible promoter PT7 control. The enzyme is thermophilic and works best at 50 C.
212152	pCT02	Plasmid expressing 6x His tagged FAST-PETase under inducible promoter PT7 control.
212153	PHB1	For PHB production in E. coli (PT7-pMB1).
212155	P48	Plasmid expressing 6x His tagged secondary alcohol dehydrogenase fused with superfolder green fluorescent protein at N terminus under constitutive promoter PgyrA control.