Chang Liu Lab: Continuous evolution of user-defined genes at 1-million-times the genomic mutation rate
Unpublished
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 213782 | pGR420 | Integrates onto p1 a partial LEU2 sequence to be subsequently used as an integration flank |
| 213783 | pGR481 | Integrates onto p1 a catalytically dead TRP5 and URA3 that can be used for selection of transversion mutations |
| 213784 | pGR475 | Integrates at the CAN1 locus the WT TP-DNAP1 and the Hygromycin resistance marker |
| 213785 | pGR595 | Integrates at the CAN1 locus the BadBoy2 TP-DNAP1 variant and the Nourseothricin resistance marker. Includes an I-SceI transient expression cassette. |
| 213786 | pGR618 | Integrates at the CAN1 locus the BB-Tv TP-DNAP1 variant and the Nourseothricin resistance marker. Includes an I-SceI transient expression cassette |
| 213787 | pGR596 | Integrates at the CAN1 locus the BadBoy3 TP-DNAP1 variant and the Nourseothricin resistance marker. Includes an I-SceI transient expression cassette |