Accurate affinity models for SH2 domains from peptide binding assays and free-energy regression
Gagoski D, Rube HT, Rastogi C, Melo L, Li X, Voleti R, Shah NH, and Bussemaker HJ
Protein Science. 2025, 34:e70317.
(Link opens in a new window)
Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 214202 | pET28-His6-SUMO-Fyn-SH2-AviTag | Bacterial expression of SH2 domain of the Fyn kinase (B- iso) with N-terminal Thrombin-cleavable 6xHis tag and SUMO and C-terminal AviTag for Streptavidin bead functionalization for binder selections |
| 214203 | pET28-His6-SUMO-Lck-SH2-AviTag | Bacterial expression of SH2 domain of the Lck kinase with N-terminal Thrombin-cleavable 6xHis tag and SUMO and C-terminal AviTag for Streptavidin bead functionalization for binder selections |
| 214204 | pET28-His6-SUMO-Blk-SH2-AviTag | Bacterial expression of SH2 domain of the Blk kinase with N-terminal Thrombin-cleavable 6xHis tag and SUMO and C-terminal AviTag for Streptavidin bead functionalization for binder selections |
| 214205 | pET28-His6-SUMO-Fgr-SH2-AviTag | Bacterial expression of SH2 domain of the Fgr kinase with N-terminal Thrombin-cleavable 6xHis tag and SUMO and C-terminal AviTag for Streptavidin bead functionalization for binder selections |
| 214206 | pET28-His6-SUMO-Hck-SH2-AviTag | Bacterial expression of SH2 domain of the Hck kinase with N-terminal Thrombin-cleavable 6xHis tag and SUMO and C-terminal AviTag for Streptavidin bead functionalization for binder selections |
| 214207 | pET28-His6-SUMO-Lyn-SH2-AviTag | Bacterial expression of SH2 domain of the Lyn kinase with N-terminal Thrombin-cleavable 6xHis tag and SUMO and C-terminal AviTag for Streptavidin bead functionalization for binder selections |
| 214208 | pET28-His6-SUMO-Yes-SH2-AviTag | Bacterial expression of SH2 domain of the Yes kinase with N-terminal Thrombin-cleavable 6xHis tag and SUMO and C-terminal AviTag for Streptavidin bead functionalization for binder selections |
| 214231 | pET28-His6-TEV-Grb2-SH2-mCherry-AviTag | Bacterial expression of SH2 domain of the Grb2 with N-terminal TEV-cleavable 6xHis tag, C-terminal mCherry, and C-terminal AviTag for Streptavidin bead functionalization for binder selections. |