A lentiviral toolkit to monitor airway epithelial cell differentiation using bioluminescence.
Orr JC, Laali A, Durrenberger PF, Lazarus KA, El Mdawar MB, Janes SM, Hynds RE
Am J Physiol Lung Cell Mol Physiol. 2024 Oct 1;327(4):L587-L599. doi: 10.1152/ajplung.00047.2024. Epub 2024 Aug 13.
(Link opens in a new window)
PubMed
(Link opens in a new window)
Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 215326 | pCCL-dNTP63Promoter-FLuc-CMV-RLuc-dsRed2 | Lentiviral mammalian vector with firefly luciferase reporter for dNTP63 expression; renilla luciferase and dsRed2 reporter under CMV promoter. |
| 215327 | pCCL-MUC5ACPromoter-FLuc-CMV-RLuc-dsRed2 | Lentiviral mammalian vector with firefly luciferase reporter for MUC5AC expression; renilla luciferase and dsRed2 reporter under CMV promoter. |
| 215328 | pCCL-FOXJ1Promoter-FLuc-CMV-RLuc-dsRed2 | Lentiviral mammalian vector with firefly luciferase reporter for FOXJ1 expression; renilla luciferase and dsRed2 reporter under CMV promoter. |
| 215329 | pCCL-NoPromoter-FLuc-CMV-RLuc-dsRed2 | Lentiviral mammalian vector with firefly luciferase gene with no upstream promoter sequence; renilla luciferase and dsRed2 reporter under CMV promoter. |