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Enhancing cap-independent translation of linear mRNA.

Golojuch S, Largey B, El-Sagheer AH, Brown T
Nat Commun. 2025 Oct 16;16(1):9205. doi: 10.1038/s41467-025-64257-6. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
225903pmRNA-EGFP-A120In vitro transcription of cap-dependently translated mRNA encoding EGFP.
225904pmRNA-mCherry-A120In vitro transcription of cap-dependently translated mRNA encoding mCherry.
225905pmRNA-iEMCV-EGFP-A120In vitro transcription of cap-independently (EMCV IRES) translated mRNA encoding EGFP.
225906pmRNA-iHCV-EGFP-A120In vitro transcription of cap-independently (HCV IRES) translated mRNA encoding EGFP.
225907pmRNA-iSyn-EGFPIn vitro transcription of cap-independently (synthetic IRES) translated mRNA encoding EGFP.

Antibodies from Article