An acidic residue within the OCT4 dimerization interface of SOX17 is necessary and sufficient to overcome its pluripotency-inducing activity.
Ho SY, Hu H, Ho DHH, Renom APS, Yeung SW, Boerner F, Weng M, Hutchins AP, Jauch R
Stem Cell Reports. 2025 Mar 11;20(3):102398. doi: 10.1016/j.stemcr.2025.102398. Epub 2025 Feb 6.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 216174 | pHAGE2-TetO-mCherry | Generate lentiviruses to act as a positive transduction control for pHAGE2-TetO vectors in pluripotency reprogramming |
| 216175 | pHAGE2-TetO-OCT4-KLF4-cMYC | Generate lentiviruses encoding for OCT4, KLF4 and c-MYC (polycistronic vector); used in conjunction with SOX vectors for pluripotency reprogramming |
| 216176 | pHAGE2-TetO-SOX17WT | Generate lentiviruses encoding for wild-type SOX17 |
| 216177 | pHAGE2-TetO-SOX17E57A | Generate lentiviruses encoding for human SOX17E57A (HMG box numbering); for use in pluripotency reprogramming |
| 216178 | pHAGE2-TetO-SOX17E57C | Generate lentiviruses encoding for human SOX17E57C (HMG box numbering); for use in pluripotency reprogramming |
| 216179 | pHAGE2-TetO-SOX17E57D | Generate lentiviruses encoding for human SOX17E57D (HMG box numbering); for use in pluripotency reprogramming |
| 216181 | pHAGE2-TetO-SOX17E57G | Generate lentiviruses encoding for human SOX17E57G (HMG box numbering); for use in pluripotency reprogramming |
| 216182 | pHAGE2-TetO-SOX17E57H | Generate lentiviruses encoding for human SOX17E57H (HMG box numbering); for use in pluripotency reprogramming |
| 216183 | pHAGE2-TetO-SOX17E57I | Generate lentiviruses encoding for human SOX17E57I (HMG box numbering); for use in pluripotency reprogramming |
| 216184 | pHAGE2-TetO-SOX17E57K | Generate lentiviruses encoding for human SOX17E57K (HMG box numbering); for use in pluripotency reprogramming |
| 216185 | pHAGE2-TetO-SOX17E57L | Generate lentiviruses encoding for human SOX17E57L (HMG box numbering); for use in pluripotency reprogramming |
| 216186 | pHAGE2-TetO-SOX17E57M | Generate lentiviruses encoding for human SOX17E57M (HMG box numbering); for use in pluripotency reprogramming |
| 216187 | pHAGE2-TetO-SOX17E57N | Generate lentiviruses encoding for human SOX17E57N (HMG box numbering); for use in pluripotency reprogramming |
| 216188 | pHAGE2-TetO-SOX17E57P | Generate lentiviruses encoding for human SOX17E57P (HMG box numbering); for use in pluripotency reprogramming |
| 216190 | pHAGE2-TetO-SOX17E57R | Generate lentiviruses encoding for human SOX17E57R (HMG box numbering); for use in pluripotency reprogramming |
| 216191 | pHAGE2-TetO-SOX17E57S | Generate lentiviruses encoding for human SOX17E57S (HMG box numbering); for use in pluripotency reprogramming |
| 216192 | pHAGE2-TetO-SOX17E57T | Generate lentiviruses encoding for human SOX17E57T (HMG box numbering); for use in pluripotency reprogramming |
| 216193 | pHAGE2-TetO-SOX17E57V | Generate lentiviruses encoding for human SOX17E57V (HMG box numbering); for use in pluripotency reprogramming |
| 216194 | pHAGE2-TetO-SOX17E57W | Generate lentiviruses encoding for human SOX17E57W(HMG box numbering); for use in pluripotency reprogramming |
| 216195 | pHAGE2-TetO-SOX17E57Y | Generate lentiviruses encoding for human SOX17E57Y (HMG box numbering); for use in pluripotency reprogramming |
| 216196 | pHAGE2-Ef1a-OCT4-3xFLAG | Overexpress human OCT4 in HEK 293T cells for use in whole cell extract EMSAs |
| 216197 | pHAGE2-Ef1a-SOX2-3xFLAG | Overexpress human SOX2 in HEK 293T cells for use in whole cell extract EMSAs |
| 216198 | pHAGE2-Ef1a-SOX17WT-3xFLAG | Overexpress wild-type human SOX17 in HEK 293T cells for use in whole cell extract EMSAs |
| 216199 | pHAGE2-Ef1a-SOX17E57D-3xFLAG | Overexpress human SOX17E57D in HEK 293T cells for use in whole cell extract EMSAs |
| 216200 | pHAGE2-Ef1a-SOX17E57P-3xFLAG | Overexpress human SOX17E57P in HEK 293T cells for use in whole cell extract EMSAs |